LOCUS       sequence-161924-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of LacZ with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX304 LacZ-V5 blast
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(135..176)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    229..253
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccagaggt tgattgtcga cttaacgcgc caccggttag cgctagctca ttactacgta
       61 gaatcgagac cgaggagagg gttagggata ggcttaccaa ccactttgta caagaaagtt
      121 ggcaaaccgg tacgcgtaga atcgagaccg aggagagggt tagggatagg cttaccttcg
      181 aaccgcgggc cctctagact cgagcggccg ccactgtgct ggatatcaac cactttgtac
      241 aagaaagctg ggtcggcgcg cccaccctta gttttttgac accagaccaa ctggtaatgg
      301 tagcgaccgg cgctcagctg taattccgcc gatactgacg ggctccagga gtcgtctcca
      361 ccaatcccca tatggaaacc gtcgatattc agccatgtgc cttcttccgc gtgcagcaga
      421 tggcgatggc tggtttccat cagttgctgt tgactgtagc ggctgatgtt gaactggaag
      481 tcgccgcgcc actggtgtgg gccataattc aattcgcgcg tcccgcagcg cagaccgttt
      541 tcgctcggga agacgtacgg ggtatacatg tctgacaatg gcagatccca gcggtcaaaa
      601 caggcggcag taaggcggtc gggatagttt tcttgcggcc ctaatccgag ccagtttacc
      661 cgctctgcta cctgcgccag ctggcagttc aggccaatcc gcgccggatg cggtgtatcg
      721 ctcgccactt caacatcaac ggtaatcgcc atttgaccac taccatcaat ccggtaggtt
//
