LOCUS       sequence-161936-        4158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GOPC, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_GOPC_WT
FEATURES             Location/Qualifiers
     source          1..4158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(64..652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(144..163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(745..1536)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     1967..1983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1967..1983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1988..2007
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1988..2006
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2024..2123
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3492..3591)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3607..3624)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3607..3623)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3616..3638)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3687..3773
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3865..3892
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4051..4068)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
       61 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      121 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      181 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      241 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      301 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      361 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      421 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      481 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      541 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      601 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      661 tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca cgttaaggga
      721 ttttggtcat gggtggctcg agggttattt gccgactacc ttggtgatct cgcctttcac
      781 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
      841 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
      901 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
      961 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     1021 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     1081 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     1141 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     1201 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     1261 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     1321 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     1381 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     1441 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     1501 ttgagtcgat acttcggcga tcaccgcttc cctcataatg tttaactttg ttttagggcg
     1561 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     1621 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca
     1681 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     1741 gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca
     1801 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggtagcag
     1861 cgaagtcgag gcatttctgt cctggctggt ctagaggagg catgatgata tatttttatc
     1921 ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg aaacagctat
     1981 gaccatgtaa tacgactcac tataggggat atcagctgga tggcaaataa tgattttatt
     2041 ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc ttataatgcc
     2101 aactttgtac aagaaagttg gcaatcaata agatttttta tgatacagag tgtgcagatc
     2161 atctaatttt gaagcaccgt catctagcgg agtttcactt gcagtgccca ggtctccatt
     2221 ttcatgtgtg tcagttactg ccttcttatt aaatccttgt aatactttga tttccccact
     2281 tgtgtctttg caactagcac cagggttacc acctccttct aactcatcaa ggtacaaacg
     2341 gtaacgatgt ccactctcat cttcatactc tacgttttca tcatcagaat ccacttcagg
     2401 agccacataa actacttcaa attcaatctc tcctctctgc tgagaaagaa tagttacagc
     2461 ttctttatgc tttgtgtccc ttaggttaac tccgttgact gccaaaatag catccccaac
     2521 gtgcagccct ccgcatctat cagcaggttg ccccggatgg atctcagaga tgaggattgg
     2581 aacaccatgt tctttcccac ctgtaattga aatgccaagg ccttcatgat cttccttaag
     2641 gaggagaact tttctaattg gaccaacacc ttggcttttc tttagggaat cttgatcatg
     2701 gcctggtggt gcttgcattg gtcgtttcaa gtcattacgt cctctgcagg ctcggatcac
     2761 agttttgtga cgatgcaaat gtatttcagc ttctaattgg ttccaaagct tatcatgagc
     2821 aggtcccttc atatctcgtc ctagcaattg tatctgttgg acccttcctg ccagttcctt
     2881 atccaagtac ttggcagcta gtctcgcccc atatacttca gcctggagaa cagctatatg
     2941 tctacgaagg gcttcattct cttttctcaa caatttcact tcagcttcaa gttgtgcttc
     3001 tttcattttt tcttttttgt ttgcctcaag ctctctttcc aattttgcct taatggtacc
     3061 agagtcagca ctttgaccag ttttagcatg aagctgcagc tgaatagagt gcagctgtaa
     3121 aagctgatca tgtacttctt tctccaaaac aactttctct gcttgggttt ctgtcagttc
     3181 agatttcaga tccaccaact gtgcctccag cttgtggttg atttgagaca cagactgggc
     3241 tttgtggcaa agctgtgcaa agcaggagct caggctggtc atcttctgtc gcccctcata
     3301 agtgatgtcc gcttggtctg gatcgatctc tcccaggagc agatccacat ccacaaaagc
     3361 tttgtcgaac tccttctcca gcacctccag ccaccggaac atggataccc cgccaggggc
     3421 ccccacggag caggaggcgc cccctgggcc ccctccggct gctgctgggc atggaccgcc
     3481 cgccgacatg gtgccaactt ttttgtacaa agttggcatt ataaaaaagc attgctcatc
     3541 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt ggggcccgag
     3601 cttaagactg gccgtcgttt tacaacgtcg tgactgggaa aacatccatg ctagcgttaa
     3661 cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg gaagactggg
     3721 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
     3781 gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
     3841 aaactgccag gcatcaaact aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     3901 tacaaactct tcctggctag cggtacgcgt attaattgcg ttgcgctcac tgcccgcttt
     3961 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4021 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4141 aggggataac gcaggaaa
//
