LOCUS       sequence-161944-        5244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAPK7, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_MAPK7_WT
FEATURES             Location/Qualifiers
     source          1..5244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(59..647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(139..158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(740..1531)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     1962..1978
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1962..1978
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1983..2002
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1983..2001
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2019..2118
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4573..4672)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4688..4705)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4688..4704)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4697..4719)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4768..4854
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4946..4973
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(5132..5149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
       61 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      121 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      181 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      241 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      301 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      361 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      421 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      481 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      541 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      601 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      661 tcttttctac ggggtctgac gctcagtgga acgacgcgta actcacgtta agggattttg
      721 gtcatgggtg gctcgagggt tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      781 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      841 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      901 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      961 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1021 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1081 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1141 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     1201 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1261 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     1321 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     1381 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     1441 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     1501 tcgatacttc ggcgatcacc gcttccctca taatgtttaa ctttgtttta gggcgactgc
     1561 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     1621 cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca
     1681 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     1741 gtgagcgcat acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg
     1801 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggt agcagcgaag
     1861 tcgaggcatt tctgtcctgg ctggtctaga ggaggcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacgg gccagagctg ccaggaaaca gctatgacca
     1981 tgtaatacga ctcactatag gggatatcag ctggatggca aataatgatt ttattttgac
     2041 tgatagtgac ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt
     2101 tgtacaagaa agttggcaac taggggtcct ggaggtcagg caggtcagcc agcagcattg
     2161 gggagtccat ctggatctca cgctgcaggg actcaatatc ggcagggttc atgccatggc
     2221 cttcgagcca gtcagcaagc agggaggctg agagagaggc tgaatctgcc tggccatcct
     2281 gtggcccatc agccacgccc atgtcgaaag actggtttaa gaattcctcc aggtcaaagc
     2341 caacaccgta gccagcccca ctgccctttg gtgtgcctga gaacacaggg ggcagggggt
     2401 cctccacctg tgacttagat agctgctggg tcacaaggtt gacatcaggt gactctgaca
     2461 tggaagactg aggggctccc ccggcgtctg ggggcggcag gccaggtggg aagtaaggca
     2521 aaactcctgg ggtgctggag ccaggcagcc cagggggtgg cacaagtgac tgggcagcca
     2581 ggaggctggt ggaggtggca atctggggtg gcgcggggac agggatgggc ccaggagggc
     2641 cagtggggtg gggtgcaggg ccagggggtg ggcaggcagg ctggggtaca gggccagagg
     2701 tagagcccgc agattgtggt tgcgggccag tgggctgggc tacagggcca ggaggaggac
     2761 tggtaggttg gactggggtt ggggttggcg ttggcgctgg ggcaggggcc ggcacagagg
     2821 tgagggctgg ggctgcgggc cgggccattc gagtccagcg ttccaacagg cttctgtcat
     2881 tgtcactgag cactagtcca gccaaggggt cagtggaggg gcccccagag gccccagccc
     2941 cccgttcctt tcgctcccgc tcctgccgcc gtttctcccg ctccttggct cgttcttgcc
     3001 gcctccgccg cttctcctcc cgctcccgct ggcgctcctg ggctgtcacc ggcttccgag
     3061 gctcaggagc ctccaggggt gcgctggggc catctctgag ccggctcctc aaagacttga
     3121 gcagggcagc tttaagggca gccttagtat tgtctgagat ggcaccatct ttctttggtg
     3181 gggcaggctc actgactggt ggaggtggct gcagggtcag atcaatggtg tcaggtgcag
     3241 ggccggggca tggtggcggg gctggtggtg gagactccat ggcacagtcc ccactgggag
     3301 cccagggact gggcatttca acatctggac agccaggctc actagccaca ggctgtagag
     3361 aaggctggaa gcggatctgt tggcggatgc cctcacgcct tgcatggaag tcctcaattt
     3421 cagccacaat ggcctcctta atgcgctccc gagtgagggc ttcgcggtca aaggcaaagt
     3481 caaagggcgg ggcacagtca ggctcatcat caggatcatg gtacttggcc aggaaagggt
     3541 ggcgaagggc agcagctgct gagatgcgag cgctgggctc aaaacgcagc atgcgaccca
     3601 gcagtgatag ggcctggcgg tcggcacctg ggtacactgt ctcccagggc acaggctggc
     3661 gtggtggcaa gctctggata taggcccgca ccctctcagc ccccacagcc tgaatcacgg
     3721 ctggtgatgg ggtacccagc accatcatga tgagctgtag ctggtgtaca tagtttttgc
     3781 ctgggaagag ctggcgccgg gccagcatct caccaaagat gcagcccaca gaccagaggt
     3841 caatagcctg tgtatactca tgcaaagaga gcatgagctc gggcgcacgg taccagcgcg
     3901 tggccacata ctcagtcatg aagtactgat gttcagcggg cgaggtgcac aggccacgag
     3961 ccataccaaa gtcaccaatc ttgagctcac agttctcatt caccaatagg ttggagggct
     4021 tcaggtcacg gtggatgacc tgagccgagt gcatgtactt caggccccgc agcagttggt
     4081 acaggaagta gcgcacgtgt tccagtgtga ggggctgtga ggagtggatg atctggtgca
     4141 ggtcgctttc catcaggtcc aggaccacgt agacagattt gaattcgcca tagggcacgg
     4201 tgggcctcag gatgtccttg atggcgatga tgttgtcgtg tttaaagtgc ttgaggatct
     4261 tcagctccct gagggtccgc ttggcattgg tcaccacatc gaaagcatta gggatcttct
     4321 tgatggccac ctgctggccg gtgaggcggc ggcgggcgga ggacaccact ccataggccc
     4381 cgttgcctat ggtctcgatg atctcgtact cgtcgcccac gtcaaaggtc acatcgaagg
     4441 agcgggcttt aagcagggcc aggttcttgg ccgctacaga ggcagcggtg tgggcgggtt
     4501 cggccttcac gggcccgggg ggctccgcag agccgtcctc gccgtcttcc tccttcagag
     4561 gctcggccat ggtgccaact tttttgtaca aagttggcat tataaaaaag cattgctcat
     4621 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tggggcccga
     4681 gcttaagact ggccgtcgtt ttacaacgtc gtgactggga aaacatccat gctagcgtta
     4741 acgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc ggaagactgg
     4801 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     4861 ggagcggatt tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     4921 taaactgcca ggcatcaaac taagcagaag gccatcctga cggatggcct ttttgcgttt
     4981 ctacaaactc ttcctggcta gcggtacgcg tattaattgc gttgcgctca ctgcccgctt
     5041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5221 caggggataa cgcaggaaag aaca
//
