LOCUS       sequence-161949-        3525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TPK1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_TPK1_WT
FEATURES             Location/Qualifiers
     source          1..3525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(547..1338)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     1769..1785
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1769..1785
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1790..1809
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1790..1808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1826..1925
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2661..2760)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2776..2793)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2776..2792)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2785..2807)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2856..2942
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3034..3061
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3220..3237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3391..3525,1..454))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3471..3490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
       61 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      121 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      181 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      241 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      301 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      361 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      421 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      481 gtctgacgct cagtggaacg acgcgtaact cacgttaagg gattttggtc atgggtggct
      541 cgagggttat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc
      601 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
      661 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
      721 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
      781 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
      841 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      901 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      961 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
     1021 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
     1081 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
     1141 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
     1201 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
     1261 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
     1321 gatcaccgct tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac
     1381 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
     1441 tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac
     1501 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     1561 ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat
     1621 ccgtttccac ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct
     1681 gtcctggctg gtctagagga ggcatgatga tatattttta tcttgtgcaa tgtaacatca
     1741 gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc
     1801 actatagggg atatcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg
     1861 ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagt
     1921 tggcaatcag cttttgatgg ccatggtcca gaggagtggg tggtcagttt ccacagtcac
     1981 aacaccagac ccgtcgtagg tattggaagt actgaccaat gttccaaaag caagcacatc
     2041 atttgtgagg ttccacttga ggcctgtggt tgtaacctgc atacaaggct gtccaacagg
     2101 aataaggcca caccaatcac cctccattcc agtgtctaca tgcaacctgt gctttcctgg
     2161 ttggagcagg tagatcagcg attcctcttg gattattata attggaaaag gagtgatgtg
     2221 agtcgcttgg aacaaggtat tcacagatgc cataatctgg tcaaaacgcc cagcaaggcc
     2281 tcccagtgtc acgatcacat caacctttaa gtctttttct tctatcttct tttggagcat
     2341 tttaaggcac ttagtaaagt cagtgtggtc ttgatcagga gttgaaatga gctcacatcc
     2401 cttagtagca tagtattctc tgacttcagg cctaatagaa tcaaagtctc cattgatgaa
     2461 ttcaggcaaa aagctttctc tctctccttc ggtgatatca tataagcggt tggcacctcc
     2521 atcggcacag gctcttaaaa gagctttgtt ccaaagatga cgaaaatagt tgtccaaagg
     2581 ctgattaaga attacaaggc agtacttcaa attcccagtg gaaagcaggg gctccaacgg
     2641 ggtaaaggca tgctccatgg tgccaacttt tttgtacaaa gttggcatta taaaaaagca
     2701 ttgctcatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2761 gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc
     2821 tagcgttaac gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg
     2881 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     2941 atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg cgggcaggac
     3001 gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg gatggccttt
     3061 ttgcgtttct acaaactctt cctggctagc ggtacgcgta ttaattgcgt tgcgctcact
     3121 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3181 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3241 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3301 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3421 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3481 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgac
//
