LOCUS       sequence-161950-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for doxycycline-inducible expression of membrane 
            targeted kinase dead CSK in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: Tet-myr-CSK K222R-GFP
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..31
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             31..72
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 atatcaacaa gtttgtacaa aaaagttggc atggggagca gcaagagcaa gcccaaggac
       61 cccagccagc gccggcgcag gatgtcagca atacaggccg cctggccatc cggtacagaa
      121 tgtattgcca agtacaactt ccacggcact gccgagcagg acctgccctt ctgcaaagga
      181 gacgtgctca ccattgtggc cgtcaccaag gaccccaact ggtacaaagc caaaaacaag
      241 gtgggccgtg agggcatcat cccagccaac tacgtccaga agcgggaggg cgtgaaggcg
      301 ggtaccaaac tcagcctcat gccttggttc cacggcaaga tcacacggga gcaggctgag
      361 cggcttctgt acccgccgga gacaggcctg ttcctggtgc gggagagcac caactacccc
      421 ggagactaca cgctgtgcgt gagctgcgac ggcaaggtgg agcactaccg catcatgtac
      481 catgccagca agctcagcat cgacgaggag gtgtactttg agaacctcat gcagctggtg
      541 gagcactaca cctcagacgc agatggactc tgtacgcgcc tcattaaacc aaaggtcatg
      601 gagggcacag tggcggccca ggatgagttc taccgcagcg gctgggccct gaacatgaag
      661 gagctgaagc tgctgcagac catcgggaag ggggagttcg gagacgtgat gctgggcgat
      721 taccgaggga acaaagtcgc cgtcaggtgc attaagaacg acgccactgc ccaggccttc
      781 ctggctgaag cctcagtcat gacgcaactg cggcatagca acctggtgca gctcctgggc
      841 gtgatcgtgg aggagaaggg cgggctctac atcgtcactg agtacatggc caaggggagc
      901 cttgtggact acctgcggtc taggggtcgg tcagtgctgg gcggagactg tctcctcaag
      961 ttctcgctag atgtctgcga ggccatggaa tacctggagg gcaacaattt cgtg
//
