LOCUS       sequence-161956-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila gRNA expression plasmid targets ebony.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Kane NS, Vora M, Varre KJ, Padgett RW
  TITLE     Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila 
            Using a Co-CRISPR Strategy.
  JOURNAL   G3 (Bethesda). 2017 Jan 5;7(1):87-93. doi: 10.1534/g3.116.036723.
  PUBMED    27793971
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2017-01-5- 5"; volume: "7"; 
            issue: "1"; pages: "87-93"
COMMENT     Sequence Label: pCFD3-ebony
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(287..362)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gttgttggtt ggcacaccac aaatatactg ttgccgagca caattgtcta gaatgcatac
       61 gcattaagcg aacattaaaa agatgtgaaa acataactat tatgtctaaa taaacacacg
      121 tcagatgtat gtacgtcaac ggaaaaccat tgtctatata ttacaattac taaatacata
      181 ccaaattgaa tacatattga tgaaaaataa taaatactgg cgaaagcaaa aaaacgaaac
      241 atttttattt tattgaacaa ctctcaggct ccaggtaggc aaaaaagcac cgactcggtg
      301 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
      361 actgacgatc gacaattgtg gcgacgttaa attgaaaata ggtctatata tacgaactga
      421 gtctggaaaa agaagttgag aattataaaa agtagtgagc actggcgccc tctctgcttg
      481 gcgagctaac cttttcgcct cttggctgag taggtggcgt ttcattctac tctgtaaaat
      541 taatgtagaa ttgaaacact atggtcaaaa aatacttagg ggcataagtt ataaaacgta
      601 tgaaatgaat ttttatcaac ctgggctatt caaaaatttt cgaattattt tatgtatttt
      661 ttttaatcgt ttttcattat aggttaaaat acactttaaa aggaattctt tcctgtaaaa
      721 taaatataaa taaatatgct ttattgacag aa
//
