LOCUS       sequence-161957-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila gRNA expression plasmid targets ebony.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Kane NS, Vora M, Varre KJ, Padgett RW
  TITLE     Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila 
            Using a Co-CRISPR Strategy.
  JOURNAL   G3 (Bethesda). 2017 Jan 5;7(1):87-93. doi: 10.1534/g3.116.036723.
  PUBMED    27793971
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2017-01-5- 5"; volume: "7"; 
            issue: "1"; pages: "87-93"
COMMENT     Sequence Label: pCFD3-ebony
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(43..463)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
ORIGIN
        1 cttgctattt ctagctctaa aactgacgat cgacaattgt ggcgacgtta aattgaaaat
       61 aggtctatat atacgaactg agtctggaaa aagaagttga gaattataaa aagtagtgag
      121 cactggcgcc ctctctgctt ggcgagctaa ccttttcgcc tcttggctga gtaggtggcg
      181 tttcattcta ctctgtaaaa ttaatgtaga attgaaacac tatggtcaaa aaatacttag
      241 gggcataagt tataaaacgt atgaaatgaa tttttatcaa cctgggctat tcaaaaattt
      301 tcgaattatt ttatgtattt tttttaatcg tttttcatta taggttaaaa tacactttaa
      361 aaggaattct ttcctgtaaa ataaatataa ataaatatgc tttattgaca gaaaatttga
      421 tgtttttgta tttgagtagg agcaatcaca ggtgagcaaa aaagaattca gatctcaatt
      481 gatcggctaa atggtatggc aagaaaaggt atgcaatata ataatctttt attgggtatg
      541 caacgaaaat ttgtttcgtc aacgtatgca atattcttta ttaaaagagg gtatgcaatg
      601 tattttatta aaaacgggta tgcaatataa taatctttta ttgggtatgc aacgaaaatt
      661 tgtttcgtca aagtatgcaa tattttttat taaaagaggg tatgcaatgt attttattaa
      721 aaacgggtat gcaataaaaa attatttggt ttctctaaaa agtatgcagc acttattttt
      781 tgataaggta tgcaacaaaa ttt
//
