LOCUS       sequence-161958-        4158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MYC, part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_MYC_WT
FEATURES             Location/Qualifiers
     source          1..4158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(588..687)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(704..723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(705..723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(728..744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(728..744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1175..1966
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2059..2647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2548..2567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2801..2818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2977..3004)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3096..3182)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3231..3253
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3245..3262
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3246..3262
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3278..3377
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(3425..4158,1..586)
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
ORIGIN
        1 gcccaccacc agcagcgact ctgaggagga acaagaagat gaggaagaaa tcgatgttgt
       61 ttctgtggaa aagaggcagg ctcctggcaa aaggtcagag tctggatcac cttctgctgg
      121 aggccacagc aaacctcctc acagcccact ggtcctcaag aggtgccacg tctccacaca
      181 tcagcacaac tacgcagcgc ctccctccac tcggaaggac tatcctgctg ccaagagggt
      241 caagttggac agtgtcagag tcctgagaca gatcagcaac aaccgaaaat gcaccagccc
      301 caggtcctcg gacaccgagg agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca
      361 gaggaggaac gagctaaaac ggagcttttt tgccctgcgt gaccagatcc cggagttgga
      421 aaacaatgaa aaggccccca aggtagttat ccttaaaaaa gccacagcat acatcctgtc
      481 cgtccaagca gaggagcaaa agctcatttc tgaagaggac ttgttgcgga aacgacgaga
      541 acagttgaaa cacaaacttg aacagctacg gaactcttgt gcgtaattgc caactttctt
      601 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
      661 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
      721 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
      781 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
      841 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
      901 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
      961 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     1021 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     1081 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     1141 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     1201 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     1261 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     1321 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     1381 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     1441 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     1501 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     1561 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     1621 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     1681 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     1741 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     1801 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     1861 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     1921 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     1981 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     2041 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2101 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2161 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2221 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2281 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2341 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2401 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2461 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2521 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2581 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2641 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2701 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2761 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2821 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2881 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
     2941 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     3001 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     3061 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     3121 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
     3181 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
     3241 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact
     3301 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
     3361 gtacaaaaaa gttggcacca tggatttttt tcgggtagtg gaaaaccagc agcctcccgc
     3421 gacgatgccc ctcaacgtta gcttcaccaa caggaactat gacctcgact acgactcggt
     3481 gcagccgtat ttctactgcg acgaggagga gaacttctac cagcagcagc agcagagcga
     3541 gctgcagccc ccggcgccca gcgaggatat ctggaagaaa ttcgagctgc tgcccacccc
     3601 gcccctgtcc cctagccgcc gctccgggct ctgctcgccc tcctacgttg cggtcacacc
     3661 cttctccctt cggggagaca acgacggcgg tggcgggagc ttctccacgg ccgaccagct
     3721 ggagatggtg accgagctgc tgggaggaga catggtgaac cagagtttca tctgcgaccc
     3781 ggacgacgag accttcatca aaaacatcat catccaggac tgtatgtgga gcggcttctc
     3841 ggccgccgcc aagctcgtct cagagaagct ggcctcctac caggctgcgc gcaaagacag
     3901 cggcagcccg aaccccgccc gcggccacag cgtctgctcc acctccagct tgtacctgca
     3961 ggatctgagc gccgccgcct cagagtgcat cgacccctcg gtggtcttcc cctaccctct
     4021 caacgacagc agctcgccca agtcctgcgc ctcgcaagac tccagcgcct tctctccgtc
     4081 ctcggattct ctgctctcct cgacggagtc ctccccgcag ggcagccccg agcccctggt
     4141 gctccatgag gagacacc
//
