LOCUS       sequence-161961-        3744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing AKR1B1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_AKR1B1_WT
FEATURES             Location/Qualifiers
     source          1..3744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     705..721
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     705..721
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     726..745
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        726..744
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    762..861
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1816..1915)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1931..1948)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1931..1947)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1940..1962)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2011..2097
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2189..2216
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2375..2392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2546..3134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2626..2645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3227..3744,1..274))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
       61 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
      121 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
      181 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
      241 gagtcgatac ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac
      301 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
      361 gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag
      421 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
      481 tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact
      541 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg
      601 aagtcgaggc atttctgtcc tggctggtct agaggaggca tgatgatata tttttatctt
      661 gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga
      721 ccatgtaata cgactcacta taggggatat cagctggatg gcaaataatg attttatttt
      781 gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttctt ataatgccaa
      841 ctttgtacaa gaaagttggc aatcaaaact cttcatggaa ggggtaatcc ttgtgggagg
      901 tacagctcaa caaggcacag accctccagt tcctgttgta gctgagtaag gtggtcatat
      961 cctggctgct cagttcaaag tcaaagacct taaagttctc agcaatgcgt tctggtgtca
     1021 cagacttggg gatcaccacc aagttcctct gcatggggaa ccggatcagg acctgggctg
     1081 tagttttatt gtgcttggct gcgatcgcct tgatcctggg atcctccagg agagaagggt
     1141 cctcgggctt ggcccagggc ctgtcaggag agccgagggg gctgtaggcg gtcaccacga
     1201 tgcctttgga ctggcagtac tggattaact tctcctgagt gagatatggg tggcactcaa
     1261 tctggttaac tgcaggctta tacttcaagc caggtttgtt taagatcatc tccacctgga
     1321 gatggttgaa gttggagatg ccaatagctt tcaccagccc ttcatccacc agctcttcca
     1381 tggccgccca cgtgtccaga atgttggtgt cactgggaac cacattgccc gactcatcca
     1441 atgggaaaaa ttccttccca ggcttaaagc cagtcggcca gtgaataagg tagaggtcca
     1501 ggtagtccag cttcaggtcg ctgagtgtct tctggcaggc tcctttcacc aggcccttct
     1561 catggtacgt gcaccacagc ttgctgacga tgaagagctc ctcacgcttc accacctgct
     1621 ccctgagctt ctcctgaatg gccaccccca cctcattctc attctggtac acatgggcac
     1681 agtcgatgtg gcggtacccg acgtcaatgg ccaccttcac ggcctcagtc acctgccctg
     1741 gaggggactt ccaggtaccc aaccccagga tgggcatctt ggcgccgttg ttgagcagga
     1801 gacggcttgc catggtgcca acttttttgt acaaagttgg cattataaaa aagcattgct
     1861 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttggggcc
     1921 cgagcttaag actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg
     1981 ttaacgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac
     2041 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     2101 ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg
     2161 ccataaactg ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg
     2221 tttctacaaa ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg
     2281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3181 acgcgtaact cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta
     3241 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
     3301 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
     3361 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     3421 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     3481 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     3541 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     3601 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     3661 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     3721 gccacggaat gatgtcgtcg tgca
//
