LOCUS       sequence-161963-        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for doxycycline-inducible expression of membrane 
            targeted kinase dead CSK in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: Tet-myr-CSK K222R-GFP
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     387..408
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      450..459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
       61 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      121 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      181 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
      241 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      301 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      361 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      421 cccggtgaac agctcctcgc ccttgctcac catggcggcc gccaggtgca gctcgtgggt
      481 tttgatgtgc tcaagctgct ctcggagctg taggaaggag ggccgcatgg cggcgtccag
      541 gtgccagcag ttcttcatga cttcatagac tgcgggcggg cagccgtcgg gggcatccat
      601 cttgtagccc ttctccaccc gagggacgac gtccttcagg ggaattcttg gataaggcac
      661 tcgcccaaag gagtagattt cccagagaag gattccgaaa ctccacacgt cagacttagt
      721 ggagaatttc ttctctctca gggcctcagg ggctgtccac ttgactggca gcttgcccgt
      781 gtcctgggtg ctggacgcct ccttggtgag accaaagtcg ctgaccttgg ccacattgtc
      841 ctcagacacc agcacattgc gggcagccag gtctcgatgc acgaaattgt tgccctccag
      901 gtattccatg gcctcgcaga catctagcga gaacttgagg agacagtctc cgcccagcac
      961 tgaccgaccc ctagaccgca ggtagtccac aaggctcccc ttggccatgt actcagtgac
     1021 gatgtagagc ccgcccttct cctccacgat cacgcccagg agctgcacca ggttgctatg
     1081 ccgcagttgc gtcatgactg aggcttcagc caggaaggcc tggg
//
