LOCUS       sequence-161965-        5217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FGFR3, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_FGFR3_p.S779R
FEATURES             Location/Qualifiers
     source          1..5217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3096..3195)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3212..3231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3213..3231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3236..3252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3236..3252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3683..4474
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4567..5155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5056..5075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc accatgggcg cccctgcctg cgccctcgcg ctctgcgtgg ccgtggccat
      721 cgtggccggc gcctcctcgg agtccttggg gacggagcag cgcgtcgtgg ggcgagcggc
      781 agaagtcccg ggcccagagc ccggccagca ggagcagttg gtcttcggca gcggggatgc
      841 tgtggagctg agctgtcccc cgcccggggg tggtcccatg gggcccactg tctgggtcaa
      901 ggatggcaca gggctggtgc cctcggagcg tgtcctggtg gggccccagc ggctgcaggt
      961 gctgaatgcc tcccacgagg actccggggc ctacagctgc cggcagcggc tcacgcagcg
     1021 cgtactgtgc cacttcagtg tgcgggtgac agacgctcca tcctcgggag atgacgaaga
     1081 cggggaggac gaggctgagg acacaggtgt ggacacaggg gccccttact ggacacggcc
     1141 cgagcggatg gacaagaagc tgctggccgt gccggccgcc aacaccgtcc gcttccgctg
     1201 cccagccgct ggcaacccca ctccctccat ctcctggctg aagaacggca gggagttccg
     1261 cggcgagcac cgcattggag gcatcaagct gcggcatcag cagtggagcc tggtcatgga
     1321 aagcgtggtg ccctcggacc gcggcaacta cacctgcgtc gtggagaaca agtttggcag
     1381 catccggcag acgtacacgc tggacgtgct ggagcgctcc ccgcaccggc ccatcctgca
     1441 ggcggggctg ccggccaacc agacggcggt gctgggcagc gacgtggagt tccactgcaa
     1501 ggtgtacagt gacgcacagc cccacatcca gtggctcaag cacgtggagg tgaacggcag
     1561 caaggtgggc ccggacggca caccctacgt taccgtgctc aagacggcgg gcgctaacac
     1621 caccgacaag gagctagagg ttctctcctt gcacaacgtc acctttgagg acgccgggga
     1681 gtacacctgc ctggcgggca attctattgg gttttctcat cactctgcgt ggctggtggt
     1741 gctgccagcc gaggaggagc tggtggaggc tgacgaggcg ggcagtgtgt atgcaggcat
     1801 cctcagctac ggggtgggct tcttcctgtt catcctggtg gtggcggctg tgacgctctg
     1861 ccgcctgcgc agccccccca agaaaggcct gggctccccc accgtgcaca agatctcccg
     1921 cttcccgctc aagcgacagg tgtccctgga gtccaacgcg tccatgagct ccaacacacc
     1981 actggtgcgc atcgcaaggc tgtcctcagg ggagggcccc acgctggcca atgtctccga
     2041 gctcgagctg cctgccgacc ccaaatggga gctgtctcgg gcccggctga ccctgggcaa
     2101 gccccttggg gagggctgct tcggccaggt ggtcatggcg gaggccatcg gcattgacaa
     2161 ggaccgggcc gccaagcctg tcaccgtagc cgtgaagatg ctgaaagacg atgccactga
     2221 caaggacctg tcggacctgg tgtctgagat ggagatgatg aagatgatcg ggaaacacaa
     2281 aaacatcatc aacctgctgg gcgcctgcac gcagggcggg cccctgtacg tgctggtgga
     2341 gtacgcggcc aagggtaacc tgcgggagtt tctgcgggcg cggcggcccc cgggcctgga
     2401 ctactccttc gacacctgca agccgcccga ggagcagctc accttcaagg acctggtgtc
     2461 ctgtgcctac caggtggccc ggggcatgga gtacttggcc tcccagaagt gcatccacag
     2521 ggacctggct gcccgcaatg tgctggtgac cgaggacaac gtgatgaaga tcgcagactt
     2581 cgggctggcc cgggacgtgc acaacctcga ctactacaag aagacaacca acggccggct
     2641 gcccgtgaag tggatggcgc ctgaggcctt gtttgaccga gtctacactc accagagtga
     2701 cgtctggtcc tttggggtcc tgctctggga gatcttcacg ctggggggct ccccgtaccc
     2761 cggcatccct gtggaggagc tcttcaagct gctgaaggag ggccaccgca tggacaagcc
     2821 cgccaactgc acacacgacc tgtacatgat catgcgggag tgctggcatg ccgcgccctc
     2881 ccagaggccc accttcaagc agctggtgga ggacctggac cgtgtcctta ccgtgacgtc
     2941 caccgacgag tacctggacc tgtcggcgcc tttcgagcag tactccccgg gtggccagga
     3001 cacccccagg tccagctcct caggggacga ctccgtgttt gcccacgacc tgctgccccc
     3061 ggccccaccc agcagtgggg gctcgcggac gtgattgcca actttcttgt acaaagttgg
     3121 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     3181 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     3241 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3301 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     3361 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     3421 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     3481 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3541 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     3601 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     3661 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     3721 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     3781 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3841 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     3901 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     3961 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     4021 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     4081 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     4141 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     4201 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     4261 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     4321 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     4381 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     4441 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     4501 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4561 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4621 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4681 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4741 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4801 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4861 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4921 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4981 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5041 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5101 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5161 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
