LOCUS       sequence-161967-        5580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RB1 , part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RB1_p.A772S
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3459..3558)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3575..3594)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3576..3594)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3599..3615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3599..3615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4046..4837
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4930..5518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5419..5438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgccgccca aaaccccccg aaaaacggcc gccaccgccg ccgctgccgc
      721 cgcggaaccc ccggcaccgc cgccgccgcc ccctcctgag gaggacccag agcaggacag
      781 cggcccggag gacctgcctc tcgtcaggct tgagtttgaa gaaacagaag aacctgattt
      841 tactgcatta tgtcagaaat taaagatacc agatcatgtc agagagagag cttggttaac
      901 ttgggagaaa gtttcatctg tggatggagt attgggaggt tatattcaaa agaaaaagga
      961 actgtgggga atctgtatct ttattgcagc agttgaccta gatgagatgt cgttcacttt
     1021 tactgagcta cagaaaaaca tagaaatcag tgtccataaa ttctttaact tactaaaaga
     1081 aattgatacc agtaccaaag ttgataatgc tatgtcaaga ctgttgaaga agtatgatgt
     1141 attgtttgca ctcttcagca aattggaaag gacatgtgaa cttatatatt tgacacaacc
     1201 cagcagttcg atatctactg aaataaattc tgcattggtg ctaaaagttt cttggatcac
     1261 atttttatta gctaaagggg aagtattaca aatggaagat gatctggtga tttcatttca
     1321 gttaatgcta tgtgtccttg actattttat taaactctca cctcccatgt tgctcaaaga
     1381 accatataaa acagctgtta tacccattaa tggttcacct cgaacaccca ggcgaggtca
     1441 gaacaggagt gcacggatag caaaacaact agaaaatgat acaagaatta ttgaagttct
     1501 ctgtaaagaa catgaatgta atatagatga ggtgaaaaat gtttatttca aaaattttat
     1561 accttttatg aattctcttg gacttgtaac atctaatgga cttccagagg ttgaaaatct
     1621 ttctaaacga tacgaagaaa tttatcttaa aaataaagat ctagatgcaa gattattttt
     1681 ggatcatgat aaaactcttc agactgattc tatagacagt tttgaaacac agagaacacc
     1741 acgaaaaagt aaccttgatg aagaggtgaa tgtaattcct ccacacactc cagttaggac
     1801 tgttatgaac actatccaac aattaatgat gattttaaat tcagcaagtg atcaaccttc
     1861 agaaaatctg atttcctatt ttaacaactg cacagtgaat ccaaaagaaa gtatactgaa
     1921 aagagtgaag gatataggat acatctttaa agagaaattt gctaaagctg tgggacaggg
     1981 ttgtgtcgaa attggatcac agcgatacaa acttggagtt cgcttgtatt accgagtaat
     2041 ggaatccatg cttaaatcag aagaagaacg attatccatt caaaatttta gcaaacttct
     2101 gaatgacaac atttttcata tgtctttatt ggcgtgcgct cttgaggttg taatggccac
     2161 atatagcaga agtacatctc agaatcttga ttctggaaca gatttgtctt tcccatggat
     2221 tctgaatgtg cttaatttaa aagcctttga tttttacaaa gtgatcgaaa gttttatcaa
     2281 agcagaaggc aacttgacaa gagaaatgat aaaacattta gaacgatgtg aacatcgaat
     2341 catggaatcc cttgcatggc tctcagattc acctttattt gatcttatta aacaatcaaa
     2401 ggaccgagaa ggaccaactg atcaccttga atctgcttgt cctcttaatc ttcctctcca
     2461 gaataatcac actgcagcag atatgtatct ttctcctgta agatctccaa agaaaaaagg
     2521 ttcaactacg cgtgtaaatt ctactgcaaa tgcagagaca caagcaacct cagccttcca
     2581 gacccagaag ccattgaaat ctacctctct ttcactgttt tataaaaaag tgtatcggct
     2641 agcctatctc cggctaaata cactttgtga acgccttctg tctgagcacc cagaattaga
     2701 acatatcatc tggacccttt tccagcacac cctgcagaat gagtatgaac tcatgagaga
     2761 caggcatttg gaccaaatta tgatgtgttc catgtatggc atatgcaaag tgaagaatat
     2821 agaccttaaa ttcaaaatca ttgtaacagc atacaaggat cttcctcatg ctgttcagga
     2881 gacattcaaa cgtgttttga tcaaagaaga ggagtatgat tctattatag tattctataa
     2941 ctcggtcttc atgcagagac tgaaaacaaa tattttgcag tattcttcca ccaggccccc
     3001 taccttgtca ccaatacctc acattcctcg aagcccttac aagtttccta gttcaccctt
     3061 acggattcct ggagggaaca tctatatttc acccctgaag agtccatata aaatttcaga
     3121 aggtctgcca acaccaacaa aaatgactcc aagatcaaga atcttagtat caattggtga
     3181 atcattcggg acttctgaga agttccagaa aataaatcag atggtatgta acagcgaccg
     3241 tgtgctcaaa agaagtgctg aaggaagcaa ccctcctaaa ccactgaaaa aactacgctt
     3301 tgatattgaa ggatcagatg aagcagatgg aagtaaacat ctcccaggag agtccaaatt
     3361 tcagcagaaa ctggcagaaa tgacttctac tcgaacacga atgcaaaagc agaaaatgaa
     3421 tgatagcatg gatacctcaa acaaggaaga gaaatgattg ccaactttct tgtacaaagt
     3481 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     3541 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     3601 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     3661 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     3721 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     3781 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     3841 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     3901 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     3961 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     4021 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     4081 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     4141 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     4201 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     4261 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     4321 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     4381 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     4441 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     4501 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     4561 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     4621 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     4681 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     4741 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     4801 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     4861 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4921 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4981 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5041 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5101 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5161 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5221 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5281 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5341 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5401 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5461 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5521 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
