LOCUS       sequence-161969-        4071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM34, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RBM34_WT
FEATURES             Location/Qualifiers
     source          1..4071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(109..697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(189..208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(790..1581)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2012..2028
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2012..2028
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2033..2052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2033..2051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2069..2168
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3450..3549)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3565..3582)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3565..3581)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3574..3596)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3645..3731
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3823..3850
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4009..4026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
       61 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      121 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      181 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      241 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      301 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      361 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      421 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      481 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      541 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      601 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      661 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      721 ggggtctgac gctcagtgga acgacgcgta actcacgtta agggattttg gtcatgggtg
      781 gctcgagggt tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
      841 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
      901 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
      961 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     1021 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     1081 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     1141 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     1201 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
     1261 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     1321 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
     1381 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     1441 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     1501 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     1561 ggcgatcacc gcttccctca taatgtttaa ctttgtttta gggcgactgc cctgctgcgt
     1621 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     1681 ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc
     1741 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     1801 acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt
     1861 catccgtttc cacggtgtgc gtcacccggc aaccttgggt agcagcgaag tcgaggcatt
     1921 tctgtcctgg ctggtctaga ggaggcatga tgatatattt ttatcttgtg caatgtaaca
     1981 tcagagattt tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga
     2041 ctcactatag gggatatcag ctggatggca aataatgatt ttattttgac tgatagtgac
     2101 ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt tgtacaagaa
     2161 agttggcaat catttctgtt ttctctgttt cttagggcgt ccacttttct tctgtccttt
     2221 cttcttcgtt ttaaggagaa cagctttttc tccaataaat aagcttttag gatgtccttc
     2281 tgcagttttg gaagtaaaat taagtccctg cttaggttta ctgacattct tcaatcgtgg
     2341 atttgaattt tgttgtttaa atttttcttt attaacagaa cgcatgactc tgagttttct
     2401 ccccatgagt tcagaattat ttaatttcag agcaagatga acagaatctg tattctcaaa
     2461 gagcacatag ccaaaccctt tgccgatgcc tgtcattttg tctctcacaa tcctcacggc
     2521 catgatactt ccacagtcca gaaagtgctt ctcaatggca gattcttcaa ctttataagg
     2581 gagattcccc acaaaaaccg atctcttgtc tctagatgag gtctcagatg cgagatcaac
     2641 tctaatacga aatccatctg caatctgggc cccatttctt ttcaatgctt gcgtggcagc
     2701 actctcctcc ttaaacacaa cataggcatt aatatttttc tgatcaggat gaattttacg
     2761 ttttattgct gccaactttt tggatagcgt tccctctgct ggaatcagag aacgaaatcg
     2821 tacagattct atttgtccat actctttaaa aaacgacttc agcttcttct tattacatgt
     2881 aacaggcaaa ttcccaacaa acacagttct ctcattcttt aatctctctt cttcttggtt
     2941 gatttgaatt ttctttcttt gactgacaac tgtgtcttct gtgtcatcaa gtatttttct
     3001 atctgctact ttaacaccag gttgagaatt tttccttttc tgcccttgtt tctggtgaat
     3061 ttcttcttct aaatcagcac tcgctagagc gctttccctg tctgccaact ttttttctgc
     3121 gttagtgtgt ttcttcttcg ctttcacttt tttggcaggt tcttgcgaaa gtggtctttc
     3181 aatctgggat gtactttctt cctcctcatt ccgtttcgtt tttttgatgg tttgtttagg
     3241 cacaggcacg tacacgggtt gaatctgggg ctccagagaa ctgaagaggg acgccagccg
     3301 accggtgcca cctctggaat ggtgttcgcc gcgaaataag ctactggcga cctgtccaag
     3361 cctgtagtct tccggcggac tcccgcgaac gccgtcgtca ggattctctc cctcctggac
     3421 acttctcttt ctcttccgtt tgctcatggt gccaactttt ttgtacaaag ttggcattat
     3481 aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     3541 cattatttgg ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
     3601 catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     3661 ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     3721 gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc ggagggtggc
     3781 gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc atcctgacgg
     3841 atggcctttt tgcgtttcta caaactcttc ctggctagcg gtacgcgtat taattgcgtt
     3901 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3961 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4021 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa a
//
