LOCUS       sequence-161972-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector with HA epitope fused to C-terminus of mCherry 
            without pA for imaging, epitope labeling/purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-mCherry-HA no-pA
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..169)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             172..876
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(314..332)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     582..601
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     776..799
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             883..909
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctcgaat tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatcca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca actttcttgt acaaagtggc ggtgagcaag
      181 ggcgaggagg acaacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      241 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      301 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      361 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      421 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      481 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      541 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      601 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      661 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc
      721 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagctg
      781 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc
      841 cgccactcca ccggcggcat ggacgagctg tacaaggtcg actatccgta cgacgtacca
      901 gactacgcag catagcaact t
//
