LOCUS       sequence-161978-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for doxycycline-inducible expression of FRNK in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: Tet-FRNK-GFP
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(118..630,631..633,634..636))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     311..330
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     570..591
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    662..686
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
       61 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
      121 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
      181 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      241 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      301 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
      361 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
      421 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
      481 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      541 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      601 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg gatcccatcg
      661 aaccactttg tacaagaaag ttgggtagtg gggcctggac tggctgatca ttttcagtct
      721 ggcttgatcg atgacatcca gcaagttctt ggcatccaca gccagagcgt gagcagccgt
      781 cagcatttgc ttcttgtact cctgctgcag gctggtcatg acgtactgct gggccagctt
      841 catcttgtta atgagctcag ccaggtcaga gttcagcagt ttctgggcca tctcaatctc
      901 tctgtgggtg cttgcaggaa gcactggcag cgactcatcc actgttgcta gcaaggttct
      961 cagcgccaag ccaacctcct ttaccatggg cacgtactcc tctggcggag ctggctgtat
     1021 tttactggac atctctatga cagctttcac cagcccggtt acattctcat agactttgtc
     1081 attggagcgg tccaggttgg ccgtaggagg agggctga
//
