LOCUS       sequence-161980-        4614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ARAF, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ARAF_p.V145L
FEATURES             Location/Qualifiers
     source          1..4614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2493..2592)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2609..2628)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2610..2628)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2633..2649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2633..2649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3080..3871
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3964..4552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4453..4472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggagccac cacggggccc ccctgccaat ggggccgagc catcccgggc
      721 agtgggcacc gtcaaagtat acctgcccaa caagcaacgc acggtggtga ctgtccggga
      781 tggcatgagt gtctacgact ctctagacaa ggccctgaag gtgcggggtc taaatcagga
      841 ctgctgtgtg gtctaccgac tcatcaaggg acgaaagacg gtcactgcct gggacacagc
      901 cattgctccc ctggatggcg aggagctcat tgtcgaggtc cttgaagatg tcccgctgac
      961 catgcacaat tttgtacgga agaccttctt cagcctggcg ttctgtgact tctgccttaa
     1021 gtttctgttc catggcttcc gttgccaaac ctgtggctac aagttccacc agcattgttc
     1081 ctccaaggtc cccacagtct gtcttgacat gagtaccaac cgccaacagt tctaccacag
     1141 tgtccaggat ttgtccggag gctccagaca gcatgaggct ccctcgaacc gccccctgaa
     1201 tgagttgcta accccccagg gtcccagccc ccgcacccag cactgtgacc cggagcactt
     1261 ccccttccct gccccagcca atgcccccct acagcgcatc cgctccacgt ccactcccaa
     1321 cgtccatatg gtcagcacca cggcccccat ggactccaac ctcatccagc tcactggcca
     1381 gagtttcagc actgatgctg ccggtagtag aggaggtagt gatggaaccc cccgggggag
     1441 ccccagccca gccagcgtgt cctcggggag gaagtcccca cattccaagt caccagcaga
     1501 gcagcgcgag cggaagtcct tggccgatga caagaagaaa gtgaagaacc tggggtaccg
     1561 ggactcaggc tattactggg aggtaccacc cagtgaggtg cagctgctga agaggatcgg
     1621 gacgggctcg tttggcaccg tgtttcgagg gcggtggcat ggcgatgtgg ccgtgaaggt
     1681 gctcaaggtg tcccagccca cagctgagca ggcccaggct ttcaagaatg agatgcaggt
     1741 gctcaggaag acgcgacatg tcaacatctt gctgtttatg ggcttcatga cccggccggg
     1801 atttgccatc atcacacagt ggtgtgaggg ctccagcctc taccatcacc tgcatgtggc
     1861 cgacacacgc ttcgacatgg tccagctcat cgacgtggcc cggcagactg cccagggcat
     1921 ggactacctc catgccaaga acatcatcca ccgagatctc aagtctaaca acatcttcct
     1981 acatgagggg ctcacggtga agatcggtga ctttggcttg gccacagtga agactcgatg
     2041 gagcggggcc cagcccttgg agcagccctc aggatctgtg ctgtggatgg cagctgaggt
     2101 gatccgtatg caggacccga acccctacag cttccagtca gacgtctatg cctacggggt
     2161 tgtgctctac gagcttatga ctggctcact gccttacagc cacattggct gccgtgacca
     2221 gattatcttt atggtgggcc gtggctatct gtccccggac ctcagcaaaa tctccagcaa
     2281 ctgccccaag gccatgcggc gcctgctgtc tgactgcctc aagttccagc gggaggagcg
     2341 gcccctcttc ccccagatcc tggccacaat tgagctgctg caacggtcac tccccaagat
     2401 tgagcggagt gcctcggaac cctccttgca ccgcacccag gccgatgagt tgcctgcctg
     2461 cctactcagc gcagcccgcc ttgtgccttg attgccaact ttcttgtaca aagttggcat
     2521 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2581 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2641 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     2701 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     2761 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     2821 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     2881 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     2941 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3001 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3061 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3121 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3181 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     3241 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3301 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     3361 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     3421 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     3481 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     3541 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3601 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3661 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     3721 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     3781 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     3841 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     3901 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3961 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4021 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4081 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4141 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4201 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4261 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4321 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4381 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4441 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4501 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4561 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
