LOCUS       sequence-161981-        3327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FAIM, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_FAIM_WT_V5
FEATURES             Location/Qualifiers
     source          1..3327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     269..288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     522..539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(698..725)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(817..903)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     952..974
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     966..983
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     967..983
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    999..1098
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1636..1735)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1752..1771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1753..1771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1776..1792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1776..1792)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2223..3014
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3107..3327,1..368)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
       61 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      121 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      181 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      241 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      301 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      361 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      421 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      481 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      541 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      601 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
      661 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
      721 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
      781 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
      841 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
      901 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
      961 gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac
     1021 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
     1081 tgtacaaaaa agttggcatg acagatctcg tagctgtttg ggatgttgct ttaagtgacg
     1141 gagtccacaa gatcgaattt gaacatggga ctacatcagg caaacgagta gtatatgtag
     1201 atggaaagga agagataaga aaagagtgga tgttcaaatt agtgggcaaa gaaacattct
     1261 atgttggagc tgcaaagaca aaagcgacca taaatataga cgctatcagt ggttttgctt
     1321 atgaatatac tctggaaatt aatgggaaaa gtctcaagaa gtatatggag gacagatcaa
     1381 aaaccaccaa tacttgggta ttacacatgg atggtgagaa ctttagaatt gttttggaaa
     1441 aagatgctat ggacgtatgg tgcaatggta aaaaattgga gacagcgggt gagtttgtag
     1501 atgatgggac tgaaactcac ttcagtatcg ggaaccatga ctgttacata aaggctgtca
     1561 gtagtgggaa gcggaaagaa gggattattc atactctcat tgtggataat agagaaatcc
     1621 cagagattgc aagttaccca actttcttgt acaaagttgg cattataaga aagcattgct
     1681 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1741 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1801 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     1861 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1921 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1981 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2041 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     2101 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2161 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2221 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2281 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2341 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2401 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2461 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2521 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2581 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2641 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2701 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2761 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2821 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2881 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2941 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     3001 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     3061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3241 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3301 caccgcctac atacctcgct ctgctaa
//
