LOCUS       sequence-161983-        4212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SHC1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_SHC1_WT_V5
FEATURES             Location/Qualifiers
     source          1..4212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     381..398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(557..584)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(676..762)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     811..833
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     825..842
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     826..842
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    858..957
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2380..2479)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2496..2515)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2497..2515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2520..2536)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2520..2536)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2967..3758
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3851..4212,1..227)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
       61 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      481 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
      541 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
      601 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
      661 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      721 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
      781 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
      841 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
      901 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcatga
      961 acaagctgag tggaggcggc gggcgcagga ctcgggtgga agggggccag cttgggggcg
     1021 aggagtggac ccgccacggg agctttgtca ataagcccac gcggggctgg ctgcatccca
     1081 acgacaaagt catgggaccc ggggtttcct acttggttcg gtacatgggt tgtgtggagg
     1141 tcctccagtc aatgcgtgcc ctggacttca acacccggac tcaggtcacc agggaggcca
     1201 tcagtctggt gtgtgaggct gtgccgggtg ctaagggggc gacaaggagg agaaagccct
     1261 gtagccgccc gctcagctct atcctgggga ggagtaacct gaaatttgct ggaatgccaa
     1321 tcactctcac cgtctccacc agcagcctca acctcatggc cgcagactgc aaacagatca
     1381 tcgccaacca ccacatgcaa tctatctcat ttgcatccgg cggggatccg gacacagccg
     1441 agtatgtcgc ctatgttgcc aaagaccctg tgaatcagag agcctgccac attctggagt
     1501 gtcccgaagg gcttgcccag gatgtcatca gcaccattgg ccaggccttc gagttgcgct
     1561 tcaaacaata cctcaggaac ccacccaaac tggtcacccc tcatgacagg atggctggct
     1621 ttgatggctc agcatgggat gaggaggagg aagagccacc tgaccatcag tactataatg
     1681 acttcccggg gaaggaaccc cccttggggg gggtggtaga catgaggctt cgggaaggag
     1741 ccgctccagg ggctgctcga cccactgcac ccaatgccca gacccccagc cacttgggag
     1801 ctacattgcc tgtaggacag cctgttgggg gagatccaga agtccgcaaa cagatgccac
     1861 ctccaccacc ctgtccagca ggcagagagc tttttgatga tccctcctat gtcaacgtcc
     1921 agaacctaga caaggcccgg caagcagtgg gtggtgctgg gccccccaat cctgctatca
     1981 atggcagtgc accccgggac ctgtttgaca tgaagccctt cgaagatgct cttcgcgtgc
     2041 ctccacctcc ccagtcggtg tccatggctg agcagctccg aggggagccc tggttccatg
     2101 ggaagctgag ccggcgggag gctgaggcac tgctgcagct caatggggac ttcctggtac
     2161 gggagagcac gaccacacct ggccagtatg tgctcactgg cttgcagagt gggcagccta
     2221 agcatttgct actggtggac cctgagggtg tggttcggac taaggatcac cgctttgaaa
     2281 gtgtcagtca ccttatcagc taccacatgg acaatcactt gcccatcatc tctgcgggca
     2341 gcgaactgtg tctacagcaa cctgtggagc ggaaactgtg cccaactttc ttgtacaaag
     2401 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2461 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     2521 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2581 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     2641 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     2701 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2761 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2821 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2881 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2941 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     3001 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     3061 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     3121 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     3181 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     3241 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     3301 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     3361 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     3421 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3481 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3541 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3601 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3661 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3721 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3781 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3841 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3901 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3961 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4021 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4081 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4141 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4201 agcgaacgac ct
//
