LOCUS       sequence-161986-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for doxycycline-inducible expression of membrane 
            targeted-CSK in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: Tet-myr-CSK-GFP
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     384..405
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      447..456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
       61 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      121 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      181 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
      241 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      301 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      361 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      421 ggtgaacagc tcctcgccct tgctcaccat ggcggccgcc aggtgcagct cgtgggtttt
      481 gatgtgctca agctgctctc ggagctgtag gaaggagggc cgcatggcgg cgtccaggtg
      541 ccagcagttc ttcatgactt catagactgc gggcgggcag ccgtcggggg catccatctt
      601 gtagcccttc tccacccgag ggacgacgtc cttcagggga attcttggat aaggcactcg
      661 cccaaaggag tagatttccc agagaaggat tccgaaactc cacacgtcag acttagtgga
      721 gaatttcttc tctctcaggg cctcaggggc tgtccacttg actggcagct tgcccgtgtc
      781 ctgggtgctg gacgcctcct tggtgagacc aaagtcgctg accttggcca cattgtcctc
      841 agacaccagc acattgcggg cagccaggtc tcgatgcacg aaattgttgc cctccaggta
      901 ttccatggcc tcgcagacat ctagcgagaa cttgaggaga cagtctccgc ccagcactga
      961 ccgaccccta gaccgcaggt agtccacaag gctccccttg gccatgtact cagtgacgat
     1021 gtagagcccg cccttctcct ccacgatcac gcccaggagc tgcaccaggt tgctatgccg
     1081 cagttgcgtc atgactgagg cttcagcc
//
