LOCUS       sequence-161987-        3708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing EBNA1BP2, used as an over expression
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_EBNA1BP2_WT_V5
FEATURES             Location/Qualifiers
     source          1..3708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(271..370)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(386..403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(386..402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      466..552
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      644..671
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(830..847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1001..1589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1081..1100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1682..2473)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2904..2920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2904..2920
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2925..2944
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2925..2943
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2961..3060
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgttctgagg tgctggcgcc tcagatccac cgatctccgg taccggaccc agtgtcacat
       61 cgagcctttc aacccattcc agatcccgct tgaattctgc caaacattgc ttcaggccat
      121 tcacgtcgtt cacggccttc ttcggcccct ctagcacgac attgaggcct ggcttcagaa
      181 gccctcggga aaacgcatcc tgcaactctc tgtctgtgac aagggattca tcggattccg
      241 actccgaatc cgagagcggg ggagtgtcca tgccaatttt tttgtacaaa gttggcatta
      301 taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      361 tcattatttg gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa
      421 acatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
      481 gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      541 agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg
      601 cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg
      661 gatggccttt ttgcgtttct acaaactctt cctggctagc ggtacgcgta ttaattgcgt
      721 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      781 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      841 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      901 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      961 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1021 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1081 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1141 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1201 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1261 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1321 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1381 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1441 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1501 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1561 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1621 acgctcagtg gaacgacgcg taactcacgt taagggattt tggtcatggg tggctcgagg
     1681 gttatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
     1741 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
     1801 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
     1861 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
     1921 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
     1981 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
     2041 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
     2101 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
     2161 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
     2221 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
     2281 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
     2341 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
     2401 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
     2461 ccgcttccct cataatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt
     2521 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     2581 gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
     2641 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
     2701 tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt
     2761 tccacggtgt gcgtcacccg gcaaccttgg gtagcagcga agtcgaggca tttctgtcct
     2821 ggctggtcta gaggaggcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     2881 tttgagacac gggccagagc tgccaggaaa cagctatgac catgtaatac gactcactat
     2941 aggggatatc agctggatgg caaataatga ttttattttg actgatagtg acctgttcgt
     3001 tgcaacaaat tgataagcaa tgctttctta taatgccaac tttgtacaag aaagttgggt
     3061 agtgtgttct gttcttcatc ttctctcttg ttcgttttcc aggtctctta tttgaccctt
     3121 tcttgccagg cctcttgagg cctctgccat gagctgtctt ggcccggaag ctagatacat
     3181 catcatagct ctcccgagtg ttccactttg agcctttctt ctttccacca aaaccaaact
     3241 tctggttttt ataccgtcgt ttagcactgg gccccttcct catctgctgg cctttggctc
     3301 ctgccttctt gcgctgtgcc agaggtttct gatctccctc aaggaaatcc agtttatcag
     3361 agaagccttt ctgatatttc ttaatagcat tcatcatatg ggctttctcc tgctgcctct
     3421 tctgaagaac ctccgtttgc accttcttcc cgtatttcct aagtgctcgc agttgcttag
     3481 ctttttcaga cctctccatg gcagcctgtt tagtctgcag cttctgtcga atcttctgca
     3541 tctgcagatc agatttggcc atttccgcaa aataatcagt gggtcgcttc gtagggactt
     3601 tgagctgatg gaggcggggt aagactgcaa gcactgcggc ctgggcttgg cgatagaaac
     3661 tcatctctcg ctggaagtcg tcttctggat caacagcttt ctggtcct
//
