LOCUS       sequence-161988-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-22GS-Late4 (modified).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-42
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             789..818
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacgggccct ctagaattat cgatccggag
      121 gtaccatggg tgcgcgtgcg tctggttcta aatctggctc cggctctgac tctggtagca
      181 agatcgaaga gctgttcaag aaacacaaga tcgttgccgt gctgcgtgcc aatagtgtgg
      241 aagaagcgaa aaagaaagcg ctggcggttt tcctgggcgg cgttcatctg attgaaatta
      301 cctttaccgt gccggatgcg gataccgtga ttaaggaact gagctttctg aaggaaatgg
      361 gcgcgattat tggtgcgggc accgtgacca gcgtggagca gtgccgtaaa gcggtggaaa
      421 gtggcgccga attcattgtg agtccgcacc tggacgagga aattagccaa ttttgcaagg
      481 agaagggtgt gttctatatg ccaggcgtta tgaccccgac cgaactggtg aaagccatga
      541 aactgggcca taccatctta aaactgtttc cgggtgaggt ggtgggtccg cagtttgtta
      601 aagcgatgaa aggtccgttt ccgaatgtga aatttgtgcc aaccggcggt gttaatctgg
      661 acaatgtgtg cgaatggttc aaagcgggcg tgctggccgt gggcgtgggc agcgcgttag
      721 tgaaaggcac cccggtggaa gtggcggaaa aggccaaggc gttcgttgag aagattcgtg
      781 gctgcaccga acagaaactg attagcgaag aagatctggg cagcggcagt ggcgacggcg
      841 gtcgtggtag ccgtggcggc gatggtagcg gcggtagcag cggtaacccg cgccaatcta
      901 tcaaggcgtt tccaattgtg atcaactctg acggtggtga aaagtgactc gagaagct
//
