LOCUS       sequence-161996-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-22GS-Late2 (modified).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-40
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(120..149)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(871..890)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(872..890)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggtaaatcg cttccatata ttccggcggc gcggtcggca ggatcacgcg gcgaccgctg
       61 ctaccgccgc taccatcgcc gccacggcta ccacgaccgc cgtcgccact gccgctgccc
      121 agatcttctt cgctaatcag tttctgttcg gtgcagccac gaatcttctc aacgaacgcc
      181 ttggcctttt ccgccacttc caccggggtg cctttcacta acgcgctgcc cacgcccacg
      241 gccagcacgc ccgctttgaa ccattcgcac acattgtcca gattaacacc gccggttggc
      301 acaaatttca cattcggaaa cggacctttc atcgctttaa caaactgcgg acccaccacc
      361 tcacccggaa acagttttaa gatggtatgg cccagtttca tggctttcac cagttcggtc
      421 ggggtcataa cgcctggcat atagaacaca cccttctcct tgcaaaattg gctaatttcc
      481 tcgtccaggt gcggactcac aatgaattcg gcgccacttt ccaccgcttt acggcactgc
      541 tccacgctgg tcacggtgcc cgcaccaata atcgcgccca tttccttcag aaagctcagt
      601 tccttaatca cggtatccgc atccggcacg gtaaaggtaa tttcaatcag atgaacgccg
      661 cccaggaaaa ccgccagcgc tttctttttc gcttcttcca cactattggc acgcagcacg
      721 gcaacgatct tgtgtttctt gaacagctct tcgatcttgc taccagagtc agagccggag
      781 ccagatttag aaccagacgc acgcgcaccc atggtacctc cggatcgata attctagagg
      841 gcccgtttaa acgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa
      901 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag aacct
//
