LOCUS       sequence-161998-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-22GS-Late5 (modified).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-43
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(87..116)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(838..857)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(839..857)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tattccggcg gtgcggtcgg accgctgcta ccgccgctac catcgccgcc acggctacca
       61 cgaccgccgt cgccactgcc gctgcccaga tcttcttcgc taatcagttt ctgttcggtg
      121 cagccacgaa tcttctcaac gaacgccttg gccttttccg ccacttccac cggggtgcct
      181 ttcactaacg cgctgcccac gcccacggcc agcacgcccg ctttgaacca ttcgcacaca
      241 ttgtccagat taacaccgcc ggttggcaca aatttcacat tcggaaacgg acctttcatc
      301 gctttaacaa actgcggacc caccacctca cccggaaaca gttttaagat ggtatggccc
      361 agtttcatgg ctttcaccag ttcggtcggg gtcataacgc ctggcatata gaacacaccc
      421 ttctccttgc aaaattggct aatttcctcg tccaggtgcg gactcacaat gaattcggcg
      481 ccactttcca ccgctttacg gcactgctcc acgctggtca cggtgcccgc accaataatc
      541 gcgcccattt ccttcagaaa gctcagttcc ttaatcacgg tatccgcatc cggcacggta
      601 aaggtaattt caatcagatg aacgccgccc aggaaaaccg ccagcgcttt ctttttcgct
      661 tcttccacac tattggcacg cagcacggca acgatcttgt gtttcttgaa cagctcttcg
      721 atcttgctac cagagtcaga gccggagcca gatttagaac cagacgcacg cgcacccatg
      781 gtacctccgg atcgataatt ctagagggcc cgtttaaacg ctagccagct tgggtctccc
      841 tatagtgagt cgtattaatt tcgataagcc agtaagcagt ggg
//
