LOCUS       sequence-162007-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Kong D, Gong L, Arnold E, Shanmugam S, Fort PE, Gardner TW, Abcouwer
            SF
  TITLE     Insulin-like growth factor 1 rescues R28 retinal neurons from 
            apoptotic death through ERK-mediated BimEL phosphorylation 
            independent of Akt.
  JOURNAL   Exp Eye Res. 2016 Aug 7;151:82-95. doi: 10.1016/j.exer.2016.08.002.
  PUBMED    27511131
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Eye 
            Res."; date: "2016-08-7"; volume: "151:82-95. doi"; pages: " 
            10.1016/j.exer.2016.08.002"
COMMENT     Sequence Label: pSELECT-HA-mFOXO1
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(168..194)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             245..513
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(526..737)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    774..865
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(806..825)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ctcgccgcgg cgtcggggtt ggcggccgcg ccgcccgacg gcgccggact ggaggtggtc
       61 gagttggact ggttaaactc cggcctgggc agcggccagg tacaggagcg ctgccggggc
      121 agcggctcga agtccgggtc ggtctccacc acctggggcg cttcggcagc gtaatccgga
      181 acatcgtatg ggtacatggc ggtcgacacg ccggtgatct caggtaggcg ccggtcacag
      241 cttggatctg taacggcgca gaacagaaaa cgaaacaaag acgtagagtt gagcaagcag
      301 ggtcaggcaa agcgtggaga gccggctgag tctaggtagg ctccaaggga gcgccggaca
      361 aaggcccggt ctcgacctga gctttaaact tacctagacg gcggacgcag ttcaggaggc
      421 accacaggcg ggaggcggca gaacgcgact caaccggcgt ggatggcggc ctcaggtagg
      481 gcggcgggcg cgtgaaggag agatgcgagc ccctcgaagc ttcagctgtg ttctggcggc
      541 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
      601 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
      661 ccttctctag gcacccgttc aattgccgac ccctcccccc aacttctcgg ggactgtggg
      721 cgatgtgcgc tctgcccact gacgggcacc ggagcgatcg cagatccttc gatagagaaa
      781 tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt tttgtttcgt
      841 tttgttttga tggagagcgt atgttagtta cgattcacac aaaaaaccaa cacacagatg
      901 taat
//
