LOCUS       sequence-162009-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mouse FOXO1 with truncating mutation at AA 256 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     pSELECT-HA-mFOXO1-D256
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSELECT-HA-mFOXO1-D256
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(164..190)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             241..509
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(522..733)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    770..861
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(802..821)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ccgcggcgtc ggggttggcg gccgcgccgc ccgacggcgc cggactggag gtggtcgagt
       61 tggactggtt aaactccggc ctgggcagcg gccaggtaca ggagcgctgc cggggcagcg
      121 gctcgaagtc cgggtcggtc tccaccacct ggggcgcctc ggcagcgtaa tccggaacat
      181 cgtatgggta catggcggtc gacacgccgg tgatctcagg taggcgccgg tcacagcttg
      241 gatctgtaac ggcgcagaac agaaaacgaa acaaagacgt agagttgagc aagcagggtc
      301 aggcaaagcg tggagagccg gctgagtcta ggtaggctcc aagggagcgc cggacaaagg
      361 cccggtctcg acctgagctt taaacttacc tagacggcgg acgcagttca ggaggcacca
      421 caggcgggag gcggcagaac gcgactcaac cggcgtggat ggcggcctca ggtagggcgg
      481 cgggcgcgtg aaggagagat gcgagcccct cgaagcttca gctgtgttct ggcggcaaac
      541 ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc
      601 tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt
      661 ctctaggcac ccgttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat
      721 gtgcgctctg cccactgacg ggcaccggag cgatcgcaga tccttcgata gagaaatgtt
      781 ctggcacctg cacttgcact ggggacagcc tattttgcta gtttgttttg tttcgttttg
      841 ttttgatgga gagcgtatgt tagttacgat tcacacaaaa aaccaacaca cagatgt
//
