LOCUS       sequence-162010-        3792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HAT1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_HAT1_WT_V5
FEATURES             Location/Qualifiers
     source          1..3792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(38..137)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(154..173)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(155..173)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(178..194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(178..194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             625..1416
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1509..2097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1998..2017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2251..2268
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2427..2454)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2546..2632)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2681..2703
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2695..2712
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2696..2712
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2728..2827
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gattaccggc gtgttattga acgacttgct caagagtacc caactttctt gtacaaagtt
       61 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      121 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
      181 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
      241 gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg
      301 ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
      361 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
      421 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
      481 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
      541 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
      601 cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa
      661 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
      721 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
      781 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
      841 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
      901 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
      961 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     1021 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1081 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1141 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1201 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1261 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     1321 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     1381 gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa
     1441 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1501 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1561 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1621 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1681 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1741 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1801 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1861 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1921 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1981 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2041 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2101 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2161 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2221 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2281 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2341 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     2401 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     2461 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     2521 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     2581 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     2641 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     2701 acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc
     2761 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
     2821 gttggcatgt tccgtgttga atatgcatct aaagttgatg agaactttga ctgtgtagag
     2881 gcagatgatg ttgagggcaa aattagacaa atcattccac ctggattttg cacaaacacg
     2941 aatgatttcc tttctttact ggaaaaggaa gttgatttca agccattcgg aaccttactt
     3001 catacctact cagttctcag tccaacagga ggagaaaact ttacctttca gatatataag
     3061 gctgacatga catgtagagg ctttcgagaa tatcatgaaa ggcttcagac ctttttgatg
     3121 tggtttattg aaactgctag ctttattgac gtggatgatg aaagatggca ctactttcta
     3181 gtatttgaga agtataataa ggatggagct acgctctttg cgaccgtagg ctacatgaca
     3241 gtctataatt actatgtgta cccagacaaa acccggccac gtgtaagtca gatgctgatt
     3301 ttgactccat ttcaaggtca aggccatggt gctcaacttc ttgaaacagt tcatagatac
     3361 tacactgaat ttcctacagt tcttgatatt acagcggaag atccatccaa aagctatgtg
     3421 aaattacgag actttgtgct tgtgaagctt tgtcaagatt tgccctgttt ttcccgggaa
     3481 aaattaatgc aaggattcaa tgaagatatg gcgatagagg cacaacagaa gttcaaaata
     3541 aataagcaac acgctagaag ggtttatgaa attcttcgac tactggtaac tgacatgagt
     3601 gatgccgaac aatacagaag ctacagactg gatattaaaa gaagactaat tagcccatat
     3661 aagaaaaagc agagagatct tgctaagatg agaaaatgtc tcagaccaga agaactgaca
     3721 aaccagatga accaaataga aataagcatg caacatgaac agctggaaga gagttttcag
     3781 gaactagtgg aa
//
