LOCUS       sequence-162016-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of GST-tagged N-terminal region of 
            Nematostella vectensis GW182 (aa 1-1158) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, 
            Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M
  TITLE     Conservation of miRNA-mediated silencing mechanisms across 600 
            million years of animal evolution.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6. pii: gkw792.
  PUBMED    27604873
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Sep 6. pii: gkw792."
COMMENT     Sequence Label: pMCh-1479
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tcggatctgg ttccgcgtgg atctcctgca ggcatgccta aggatgacaa gaaggaattc
       61 ctcaagtcat tgggcgttga caccctgtcc tctgaaaacg acatcaagag agcctataag
      121 aaaatagcac tccagtgtca ccctgataag aaccccagcg acacgtccgc cagaaaacaa
      181 ttcgaggagg tctctaaagc attcgagtac ctcgtccacg acgggatcaa tgacagcctg
      241 aaagattacg ataaggtttt ctttgatttc ctccgggaca tattgttcgg cagcgatggt
      301 gcttcagact tctttaccta tttttgtggt tccaagcagc acagctgtat ctccacagac
      361 accgaccctg attttagata tcaggatgac gacgaggagg aggacattga tggggaacag
      421 gagctggaaa gcctcctgga gatcgtcaag aaccgccgga agcgcgggaa gcccgtaccc
      481 gatgagtttc acgaggagaa actgcaggag ctgaagactc gactgtctca gcacaaagag
      541 gacatgaaaa aggatgggag caagaaacag gctgttaaga aggggaaagc tgtcagcgtc
      601 cctgtggcaa aacccaagcc aaagagcaaa aagcagctcc ttgctgaaca acggcggcgg
      661 gctaaagaaa tggagcagat agccatggaa ctggaggaga aaagaaaaga agaagaagaa
      721 aaggagaagc aacgcaaaca gctggaagta gagaaacaga aacgccttga ggaggagcgc
      781 atcaggtcac agaacgagga gcgaaaacgg cgggagcgag agcaaaaaga acgggagaag
      841 cagcagaaac tggatatgga gaaagaggag cgccgccggc gccagcagga ggaggagaag
      901 aaaagacgag aggaggaaga gaagagaaaa aaagtcgaga aagagaagca ggaaagggag
      961 aaggccgcaa aaaaagctgc cctccaggct gcgaaggaga aagaagaaca ggcgaaggct
     1021 gcatctaaaa atcagaatat taaaagacag cagcaacaac agcgaactga tcaacagcag
     1081 tatcctaggg aaat
//
