LOCUS       sequence-162018-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The sgRNA at the stop codon of human Brachyury(T) locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, 
            Kendziorski C, Stewart R, Thomson JA
  TITLE     Single-cell RNA-seq reveals novel regulators of human embryonic stem
            cell differentiation to definitive endoderm.
  JOURNAL   Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x.
  PUBMED    27534536
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2016-08-17"; volume: "17(1):173. doi"; pages: " 
            10.1186/s13059-016-1033-x"
COMMENT     Sequence Label: T-STOP sgRNA-1
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(390..692)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(492..511)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     815..837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     829..846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     830..846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     853..872
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        853..871
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    947..971
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
       61 gtctcttgat cagagcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
      121 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
      181 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
      241 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
      301 atccggggtc agcaccgttt ctgcggactg gctttctacg tgaaaaggat ctaggtgaag
      361 atcctttttg ataatctcat gcctgacatt tatattcccc agaacatcag gttaatggcg
      421 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc
      481 ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc
      541 gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc
      601 atccgtcgcc ccggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg
      661 ctctctcttt tataggtgta aaccttaaac tgccgtacgt ataggctgcg caactgttgg
      721 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
      781 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
      841 gccagtgaat tgtaatacga ctcactatag ggcgaattgg gccctctaga tgcatgctcg
      901 agcggccgcc agtgtgatgg atatctgcag aattcgccct ttaatgccaa ctttgtacaa
      961 gaaagctggg tctagaaaaa
//
