LOCUS       sequence-162061-        7233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses OMA-1 (1-182) in bacteria for protein purification 
            purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Kaymak E, Ryder SP
  TITLE     RNA recognition by the Caenorhabditis elegans oocyte maturation 
            determinant OMA-1.
  JOURNAL   J Biol Chem. 2013 Oct 18;288(42):30463-72. doi: 
            10.1074/jbc.M113.496547. Epub 2013 Sep 6.
  PUBMED    24014033
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.496547"; journalName: "J Biol Chem"; date: 
            "2013-10-18- 18"; volume: "288"; issue: "42"; pages: "30463-72"
COMMENT     Sequence Label: pMALac-OMA-1(1-182)
FEATURES             Location/Qualifiers
     source          1..7233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        59..136
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             137..1219
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(156..175)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1462..1467,1468..1483,1484..1490)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1498..1514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1503..1525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1587..1604
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1605..2705
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2679..2702
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2760..2780
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(3380..3397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3380..3396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3389..3411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3575..3592)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3745..3831
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3923..3950
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3969..4060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4061..4129,4130..4921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4279..4298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4963..5476)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5158..5179)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5370..5389
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5587..6175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6076..6095
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6329..6346
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6361..6503
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6429..6448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6589..6611
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6605..6796)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gaaattccga
       61 caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
      121 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
      181 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
      241 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
      301 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
      361 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
      421 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
      481 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
      541 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
      601 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
      661 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
      721 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
      781 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
      841 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
      901 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcagtagtgg gatacgacga
      961 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
     1021 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     1081 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     1141 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     1201 actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac
     1261 aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt ctggcgtcag
     1321 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
     1381 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
     1441 caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg tgtggaattg
     1501 tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt tcacgagcac
     1561 ttcaccaaca aggaccatag catatgcatc atcatcatca tcatatgaaa atcgaagaag
     1621 gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta
     1681 agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag
     1741 agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg
     1801 accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt
     1861 tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg
     1921 cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc
     1981 cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga
     2041 gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg
     2101 ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata
     2161 acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga
     2221 atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga
     2281 ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa
     2341 cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag
     2401 gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc
     2461 tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga
     2521 agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc
     2581 agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta
     2641 ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc
     2701 agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc gggatcgagg
     2761 aaaacctgta ttttcagggc gaattcatga acgttaacgg tgaaaacaac gagaagatcg
     2821 atgagcatca tctcgagagc tccctcgctg gcgttccaac tttgcccgtt tcaccactcg
     2881 atcacgctaa ggacttgtct caaactaatc cgaatgccca gattggagat ttggttactc
     2941 aaactgctaa cttgattgct atcaaaaagc agttgcttga agatattgca ttcaaccaac
     3001 atattcaatc gatgcaagtt cgcgcgattc agagttttcc ccagaacaac caggttgcac
     3061 ctccatttca acagtttgat cctagacgcc gcggattagc ccgaatgcag aaaccagaat
     3121 catacaagac tgtgatctgc caagcttggc tcgaatctaa gacttgttct ttcgcagaca
     3181 actgccggtt tgcacatgga gaggaggaac ttcgtccaac attcgtcgag ccgctgcaga
     3241 acaataagta taaaacgaaa ctctgtgaca aatatacgac tacaggactc tgcccatacg
     3301 ggaaacggtg ccttttcatt catcccgatc attaatagga attcggatcc tctagagtcg
     3361 acctgcaggc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaacccag
     3421 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3481 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcagc
     3541 ttggctgttt tggcggatga gataagattt tcagcctgat acagattaaa tcagaacgca
     3601 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
     3661 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
     3721 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3781 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     3841 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     3901 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     3961 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4021 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4081 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4141 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4201 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt tcccaatgat
     4261 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     4321 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4381 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4441 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4501 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4561 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4621 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4681 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4741 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4801 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4861 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4921 actgtcagac caagtttact catatatact ttagattgat ttaccccggt tgataatcag
     4981 aaaagcccca aaaacaggaa gattgtataa gcaaatattt aaattgtaaa cgttaatatt
     5041 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     5101 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     5161 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5221 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg
     5281 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5341 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5401 gcgctggcaa gtgtagcggt cacgctgcgc gtaaacacca cacccgccgc gcttaatgcg
     5461 ccgctacagg gcgcgtaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5521 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5701 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     5761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5941 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6001 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6241 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6301 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6361 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc
     6421 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     6481 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     6541 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6601 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     6661 cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     6721 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     6781 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     6841 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     6901 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     6961 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     7021 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     7081 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     7141 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     7201 taaggcaacc ccgccagcct agccgggtcc tca
//
