LOCUS       sequence-162065-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM
  TITLE     Activation of endogenous Cdc42 visualized in living cells.
  JOURNAL   Science. 2004 Sep 10. 305(5690):1615-9.
  PUBMED    15361624
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Sep 10. 305(5690):1615-9."
COMMENT     Sequence Label: pcDNA3-EGFP-Cdc42(wt)
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..617)
                     /codon_start=1
                     /product="human Rho family small GTPase Cdc42"
                     /label=Cdc42
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     primer_bind     complement(666..687)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1015..1034
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggtgacacta tagaataggg ccctctagat gcatgctcga gtcatagcag cacacacctg
       61 cggctcttct tcggttctgg aggctccagg gcagccaata ttgcttcgtc aaatacattc
      121 tttaggcctt tctgtgtaag tgcagaacac tccacatact tgacagcctt caggtcacgg
      181 gccagctttt cagcagtctc tggagtgata ggcttctgtt tgttcttggc aagtttctca
      241 atagtagagg ggtcatctct gagatcaatt tgagtcccaa caagcaagaa aggagtcttt
      301 ggacagtggt gagttatctc aggcacccac ttttctttca cgttttcaaa tgaagatgga
      361 gagaccactg aaaaacagac tagaaataca tctgtttgtg gataactcag cggtcgtaat
      421 ctgtcataat cctcttgccc tgcagtatca aaaagtccaa gagtatatgg ttctccacca
      481 atcataactg tgactgcata gttgtcaaaa acagtcggta catattccga tggaaatttg
      541 tttgttgtgt aggatatcag gagacatgtt ttaccaacag caccatcgcc cacaacaaca
      601 cacttaattg tctgcatgaa ttccttgtac agctcgtcca tgccgagagt gatcccggcg
      661 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      721 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      781 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      841 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
      901 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      961 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     1021 tagttgccgt cgtccttgaa ga
//
