LOCUS       sequence-162075-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Palm1-12GS-I3-01-myc-Late1 (1wa3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-07 (1wa3)
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             813..842
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cagagctctc tggctactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagaat tatcgatccg
      121 gaggtaccat gggttgtatc aagagcaagc gcaaggataa tttgaacggt tctaaatctg
      181 gctccggctc tgactctggt agcaagatcg aagagctgtt caagaaacac aagatcgttg
      241 ccgtgctgcg tgccaatagt gtggaagaag cgaaagaaaa agcgctggcg gttttcgaag
      301 gcggcgttca tctgattgaa attaccttta ccgtgccgga tgcggatacc gtgattaagg
      361 aactgagctt tctgaaggaa aaaggcgcga ttattggtgc gggcaccgtg accagcgtgg
      421 agcagtgccg taaagcggtg gaaagtggcg ccgaattcat tgtgagtccg cacctggacg
      481 aggaaattag ccaattttgc aaggagaagg gtgtgttcta tatgccaggc gttatgaccc
      541 cgaccgaact ggtgaaagcc atgaaactgg gccataccat cttaaaactg tttccgggtg
      601 aggtggtggg tccgcagttt gttaaagcga tgaaaggtcc gtttccgaat gtgaaatttg
      661 tgccaaccgg cggtgttaat ctggacaatg tgtgcgaatg gttcaaagcg ggcgtgctgg
      721 ccgtgggcgt gggcagcgcg ttagtgaaag gcaccccgga tgaagtgcgt gaaaaggcca
      781 aggcgttcgt tgagaagatt cgtggctgca ccgaacagaa actgattagc gaagaagatc
      841 tgcagtctcg tccggaaccg accgcaccgc cggaagaatc tttccgttct ggtgttgaaa
      901 ccaccacgcc accgcaaaaa caggagccga tcgacaaaga actgtacccg ctgacctctc
      961 tgcgttctct gttcggtaac gacccgtctt ctcagtgact cgagaagct
//
