LOCUS       sequence-162084-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of CFP 
            and HXGPRT selectable marker cassette for endogenous tagging on the 
            gene locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pCFP.HXGPRT
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cggccgctct agaactagtg gatccccctc caccgcggtg tcactgtagc ctgccagaac
       61 acttgtcaac cgactgtgtc cacattttta tgcgcactga ctggcatgaa tggccagagg
      121 caggcatcag caagtcacgt aggccaacgc gtgcgcagaa acgctcaagg ctcgattgtg
      181 ggtgggggtt ggtagcattt tatcgaccta aacaaggttt acacttaggt ggtgcggttt
      241 tactgatctg gacggattca gcggtcgcag attatcgatc tgcaaatggt gtacacttag
      301 gtgtcgcggc ttatctagtt aagggagctt cgtggtcgga gcctaacaag tcaacagaga
      361 cgtatcgcca atcgttcgcg gtgaagagtc gaaactgaca gcacatcgta gggaaactga
      421 gagggtgctc ctttctctcc gtcgtttgcg ctgcaccatc ctgcaagtgc atagaaggaa
      481 agttgtctgc tgtcgtgggc agacagcaac agtccagcac tctagcggca tacagaacga
      541 taacgcattc acgagtggat acacgcacat ctgcgtcacc cgcaactcgc tttcgttctg
      601 attgacaaaa agaaaacaag gcgaggtgag actgtgtgaa atgccacatg aagagtcatc
      661 ccttttcttc gataaaggac acaggggtct ctggcacccc ctcgtcagct ctctccgacc
      721 cgaggcactc tccctgatcc ctccgaaaag agaggaaaac gagagacggg cagcttctgt
      781 gggctatgca gggtttactt ctcgaacttt ttgcgagcgg cgtcgctcag gacggcgacg
      841 tggtcgaagt cgcggaacat ctcgttgaag tcgtagcagc aaccaacgat ccagacgtct
      901 tcaatgctga agccgacgaa gtcgccc
//
