LOCUS       sequence-162126-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-22GS-Late9 (modified).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-47
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(78..107)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(829..848)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(830..848)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(893..944)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(922..942)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aggtccgggt aaccgctgct accgccgcta ccatcgccgc cacggctacc acgaccgccg
       61 tcgccactgc cgctgcccag atcttcttcg ctaatcagtt tctgttcggt gcagccacga
      121 atcttctcaa cgaacgcctt ggccttttcc gccacttcca ccggggtgcc tttcactaac
      181 gcgctgccca cgcccacggc cagcacgccc gctttgaacc attcgcacac attgtccaga
      241 ttaacaccgc cggttggcac aaatttcaca ttcggaaacg gacctttcat cgctttaaca
      301 aactgcggac ccaccacctc acccggaaac agttttaaga tggtatggcc cagtttcatg
      361 gctttcacca gttcggtcgg ggtcataacg cctggcatat agaacacacc cttctccttg
      421 caaaattggc taatttcctc gtccaggtgc ggactcacaa tgaattcggc gccactttcc
      481 accgctttac ggcactgctc cacgctggtc acggtgcccg caccaataat cgcgcccatt
      541 tccttcagaa agctcagttc cttaatcacg gtatccgcat ccggcacggt aaaggtaatt
      601 tcaatcagat gaacgccgcc caggaaaacc gccagcgctt tctttttcgc ttcttccaca
      661 ctattggcac gcagcacggc aacgatcttg tgtttcttga acagctcttc gatcttgcta
      721 ccagagtcag agccggagcc agatttagaa ccagacgcac gcgcacccat ggtacctccg
      781 gatcgataat tctagagggc ccgtttaaac gctagccagc ttgggtctcc ctatagtgag
      841 tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc
      901 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg ggcgga
//
