LOCUS       sequence-162128-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-22GS-Late8 (modified).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-46
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             788..817
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(935..954)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acgggccctc tagaattatc gatccggagg
      121 taccatgggt gcgcgtgcgt ctggttctaa atctggctcc ggctctgact ctggtagcaa
      181 gatcgaagag ctgttcaaga aacacaagat cgttgccgtg ctgcgtgcca atagtgtgga
      241 agaagcgaaa aagaaagcgc tggcggtttt cctgggcggc gttcatctga ttgaaattac
      301 ctttaccgtg ccggatgcgg ataccgtgat taaggaactg agctttctga aggaaatggg
      361 cgcgattatt ggtgcgggca ccgtgaccag cgtggagcag tgccgtaaag cggtggaaag
      421 tggcgccgaa ttcattgtga gtccgcacct ggacgaggaa attagccaat tttgcaagga
      481 gaagggtgtg ttctatatgc caggcgttat gaccccgacc gaactggtga aagccatgaa
      541 actgggccat accatcttaa aactgtttcc gggtgaggtg gtgggtccgc agtttgttaa
      601 agcgatgaaa ggtccgtttc cgaatgtgaa atttgtgcca accggcggtg ttaatctgga
      661 caatgtgtgc gaatggttca aagcgggcgt gctggccgtg ggcgtgggca gcgcgttagt
      721 gaaaggcacc ccggtggaag tggcggaaaa ggccaaggcg ttcgttgaga agattcgtgg
      781 ctgcaccgaa cagaaactga ttagcgaaga agatctgggc agcggcagtg gcgacggcgg
      841 tcgtggtagc cgtggcggcg atggtagcgg cggtagcagc ggttacccac tgacctccct
      901 gggtggttct tgactcgaga agcttgctag cagatctttt tccctctgcc aaaaattatg
      961 gggacatcat gaa
//
