LOCUS       sequence-162129-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-22GS-Late8 (modified).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-46
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..109)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(831..850)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(832..850)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(895..940)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 aggtcagtgg gtaaccgctg ctaccgccgc taccatcgcc gccacggcta ccacgaccgc
       61 cgtcgccact gccgctgccc agatcttctt cgctaatcag tttctgttcg gtgcagccac
      121 gaatcttctc aacgaacgcc ttggcctttt ccgccacttc caccggggtg cctttcacta
      181 acgcgctgcc cacgcccacg gccagcacgc ccgctttgaa ccattcgcac acattgtcca
      241 gattaacacc gccggttggc acaaatttca cattcggaaa cggacctttc atcgctttaa
      301 caaactgcgg acccaccacc tcacccggaa acagttttaa gatggtatgg cccagtttca
      361 tggctttcac cagttcggtc ggggtcataa cgcctggcat atagaacaca cccttctcct
      421 tgcaaaattg gctaatttcc tcgtccaggt gcggactcac aatgaattcg gcgccacttt
      481 ccaccgcttt acggcactgc tccacgctgg tcacggtgcc cgcaccaata atcgcgccca
      541 tttccttcag aaagctcagt tccttaatca cggtatccgc atccggcacg gtaaaggtaa
      601 tttcaatcag atgaacgccg cccaggaaaa ccgccagcgc tttctttttc gcttcttcca
      661 cactattggc acgcagcacg gcaacgatct tgtgtttctt gaacagctct tcgatcttgc
      721 taccagagtc agagccggag ccagatttag aaccagacgc acgcgcaccc atggtacctc
      781 cggatcgata attctagagg gcccgtttaa acgctagcca gcttgggtct ccctatagtg
      841 agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct
      901 gcttatatag aacctcccac cgtacacgcc taccgcccat
//
