LOCUS       sequence-162134-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-22GS-Late7 (modified).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-45
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             790..819
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacgggccc tctagaatta tcgatccgga
      121 ggtaccatgg gtgcgcgtgc gtctggttct aaatctggct ccggctctga ctctggtagc
      181 aagatcgaag agctgttcaa gaaacacaag atcgttgccg tgctgcgtgc caatagtgtg
      241 gaagaagcga aaaagaaagc gctggcggtt ttcctgggcg gcgttcatct gattgaaatt
      301 acctttaccg tgccggatgc ggataccgtg attaaggaac tgagctttct gaaggaaatg
      361 ggcgcgatta ttggtgcggg caccgtgacc agcgtggagc agtgccgtaa agcggtggaa
      421 agtggcgccg aattcattgt gagtccgcac ctggacgagg aaattagcca attttgcaag
      481 gagaagggtg tgttctatat gccaggcgtt atgaccccga ccgaactggt gaaagccatg
      541 aaactgggcc ataccatctt aaaactgttt ccgggtgagg tggtgggtcc gcagtttgtt
      601 aaagcgatga aaggtccgtt tccgaatgtg aaatttgtgc caaccggcgg tgttaatctg
      661 gacaatgtgt gcgaatggtt caaagcgggc gtgctggccg tgggcgtggg cagcgcgtta
      721 gtgaaaggca ccccggtgga agtggcggaa aaggccaagg cgttcgttga gaagattcgt
      781 ggctgcaccg aacagaaact gattagcgaa gaagatctgg gcagcggcag tggcgacggc
      841 ggtcgtggta gccgtggcgg cgatggtagc ggcggtagca gcggtccacc ggaatatggt
      901 ggctcctgac tcgagaagct tgctagcaga tctttt
//
