LOCUS       sequence-162135-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-22GS-Late7 (modified).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-45
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(84..113)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(835..854)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(836..854)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(899..943)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 ccaccatatt ccggtggacc gctgctaccg ccgctaccat cgccgccacg gctaccacga
       61 ccgccgtcgc cactgccgct gcccagatct tcttcgctaa tcagtttctg ttcggtgcag
      121 ccacgaatct tctcaacgaa cgccttggcc ttttccgcca cttccaccgg ggtgcctttc
      181 actaacgcgc tgcccacgcc cacggccagc acgcccgctt tgaaccattc gcacacattg
      241 tccagattaa caccgccggt tggcacaaat ttcacattcg gaaacggacc tttcatcgct
      301 ttaacaaact gcggacccac cacctcaccc ggaaacagtt ttaagatggt atggcccagt
      361 ttcatggctt tcaccagttc ggtcggggtc ataacgcctg gcatatagaa cacacccttc
      421 tccttgcaaa attggctaat ttcctcgtcc aggtgcggac tcacaatgaa ttcggcgcca
      481 ctttccaccg ctttacggca ctgctccacg ctggtcacgg tgcccgcacc aataatcgcg
      541 cccatttcct tcagaaagct cagttcctta atcacggtat ccgcatccgg cacggtaaag
      601 gtaatttcaa tcagatgaac gccgcccagg aaaaccgcca gcgctttctt tttcgcttct
      661 tccacactat tggcacgcag cacggcaacg atcttgtgtt tcttgaacag ctcttcgatc
      721 ttgctaccag agtcagagcc ggagccagat ttagaaccag acgcacgcgc acccatggta
      781 cctccggatc gataattcta gagggcccgt ttaaacgcta gccagcttgg gtctccctat
      841 agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag
      901 ctctgcttat atagacctcc caccgtacac gcctacggcc cattt
//
