LOCUS       sequence-162143-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-Late2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-36
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..88)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(810..829)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(811..829)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(874..926)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(904..924)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gaactgggta aatcgcttcc atatattccg gcggcgcggt cggcaggatc acgcggcgca
       61 gatcttcttc gctaatcagt ttctgttcgg tgcagccacg aatcttctca acgaacgcct
      121 tggccttttc cgccacttcc accggggtgc ctttcactaa cgcgctgccc acgcccacgg
      181 ccagcacgcc cgctttgaac cattcgcaca cattgtccag attaacaccg ccggttggca
      241 caaatttcac attcggaaac ggacctttca tcgctttaac aaactgcgga cccaccacct
      301 cacccggaaa cagttttaag atggtatggc ccagtttcat ggctttcacc agttcggtcg
      361 gggtcataac gcctggcata tagaacacac ccttctcctt gcaaaattgg ctaatttcct
      421 cgtccaggtg cggactcaca atgaattcgg cgccactttc caccgcttta cggcactgct
      481 ccacgctggt cacggtgccc gcaccaataa tcgcgcccat ttccttcaga aagctcagtt
      541 ccttaatcac ggtatccgca tccggcacgg taaaggtaat ttcaatcaga tgaacgccgc
      601 ccaggaaaac cgccagcgct ttctttttcg cttcttccac actattggca cgcagcacgg
      661 caacgatctt gtgtttcttg aacagctctt cgatcttgct accagagtca gagccggagc
      721 cagatttaga accagacgca cgcgcaccca tggtacctcc ggatcgataa ttctagaggg
      781 cccgtttaaa cgctagccag cttgggtctc cctatagtga gtcgtattaa tttcgataag
      841 ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga acctcccacc
      901 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
      961 cc
//
