LOCUS       sequence-162149-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recombinant AAV vector packaging single-stranded Firefly Luciferase 
            under the CAG promoter (includes WPRE element).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Pekrun K, De Alencastro G, Luo QJ, Liu J, Kim Y, Nygaard S, Galivo 
            F, Zhang F, Song R, Tiffany MR, Xu J, Hebrok M, Grompe M, Kay MA
  TITLE     Using a barcoded AAV capsid library to select for clinically 
            relevant gene therapy vectors.
  JOURNAL   JCI Insight. 2019 Nov 14;4(22). pii: 131610. doi: 
            10.1172/jci.insight.131610.
  PUBMED    31723052
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2019-11-14"; volume: "4(22). pii: 131610. doi"; 
            pages: " 10.1172/jci.insight.131610"
COMMENT     Sequence Label: pAAV-CAG-FLuc
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagaattgga tccgccacca tggaagatgc caaaaacatt aggaagggcc cagcgccatt
       61 ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc
      121 cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc
      181 cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac
      241 aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg
      301 tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct
      361 gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca
      421 aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag
      481 caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc
      541 cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct
      601 gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac
      661 cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga
      721 caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg
      781 ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt
      841 gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt
      901 cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag
      961 cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc
     1021 aggcatccgc cagggctacg gcctgac
//
