LOCUS       sequence-162174-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Cannon MB, Remington SJ
  TITLE     Re-engineering redox-sensitive green fluorescent protein for 
            improved response rate.
  JOURNAL   Protein Sci. 2006 Jan;15(1):45-57. Epub 2005 Dec 1.
  PUBMED    16322566
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2006-01"; volume: "15(1)"; pages: "45-57. Epub 2005 
            Dec 1"
COMMENT     Sequence Label: pRSETB-roGFP1-R12
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..88
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(92..100,101..115)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             134..847
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHKVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLKTCSALSKDPNEKRDHMVLLE
                     RVTAAGITHGMDELYK"
     primer_bind     complement(164..192)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc atggtatggc
       61 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc
      121 ccccgctgaa ttcatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt
      181 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      241 tgcaacatac ggaaaactta cccttaaatt tatttccact actggaaaac tacctgttcc
      301 atggccaaca cttgtcacta ctttcagtta tggtgttcaa tgcttttcaa gatacccaga
      361 tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      421 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      481 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      541 tcttggacac aaattggaat acaactataa ctgccacaag gtatacatca tggcagacaa
      601 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      661 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      721 agacaaccat tacctgaaga catgctctgc cctttcgaaa gatcccaacg aaaagagaga
      781 ccacatggtc cttcttgagc gcgtaacagc tgctgggatt acacatggca tggatgaact
      841 atacaaataa
//
