LOCUS       sequence-162175-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Cannon MB, Remington SJ
  TITLE     Re-engineering redox-sensitive green fluorescent protein for 
            improved response rate.
  JOURNAL   Protein Sci. 2006 Jan;15(1):45-57. Epub 2005 Dec 1.
  PUBMED    16322566
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2006-01"; volume: "15(1)"; pages: "45-57. Epub 2005 
            Dec 1"
COMMENT     Sequence Label: pRSETB-roGFP1-R12
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(75..788)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHKVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLKTCSALSKDPNEKRDHMVLLE
                     RVTAAGITHGMDELYK"
     primer_bind     730..758
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(join(807..821,822..830))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(834..866)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(870..887)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggctttgtta gcagccggat caagcttcga attccatggt accagctgca gatctcgagc
       61 tcggatcctt attatttgta tagttcatcc atgccatgtg taatcccagc agctgttacg
      121 cgctcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagagcat
      181 gtcttcaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt
      241 tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat tttgaagtta
      301 actttgattc cattcttttg tttgtctgcc atgatgtata ccttgtggca gttatagttg
      361 tattccaatt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ttttaactcg
      421 attctattaa caagggtatc accttcaaac ttgacttcag cacgtgtctt gtagttcccg
      481 tcatctttga aaaatatagt tctttcctgt acataacctt cgggcatggc actcttgaaa
      541 aagtcatgcc gtttcatatg atctgggtat cttgaaaagc attgaacacc ataactgaaa
      601 gtagtgacaa gtgttggcca tggaacaggt agttttccag tagtggaaat aaatttaagg
      661 gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca
      721 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct
      781 ttactcatga attcagcggg gggatcctta tcgtcatcgt cgtacagatc ccgacccatt
      841 tgctgtccac cagtcatgct agccatacca tgatgatgat gatgatgaga
//
