LOCUS       sequence-162178-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Lohman JR, Remington SJ
  TITLE     Development of a family of redox-sensitive green fluorescent protein
            indicators for use in relatively oxidizing subcellular environments.
  JOURNAL   Biochemistry. 2008 Aug 19;47(33):8678-88. doi: 10.1021/bi800498g. 
            Epub 2008 Jul 25.
  PUBMED    18652491
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi800498g";
            journalName: "Biochemistry"; date: "2008-08-19- 19"; volume: "47"; 
            issue: "33"; pages: "8678-88"
COMMENT     Sequence Label: pQE30-roGFP1-iE
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..756
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCESNVYIMADKQKNGIK
                     ANFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(70..98)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     696..716
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 actatgagag gatcgcatca ccatcaccat cacggatcca tgagtaaagg agaagaactt
       61 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      121 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      181 tccactactg gaaaactacc tgttccatgg ccaacacttg tcactactct gagttatggt
      241 gttcaatgct tttcaagata cccagatcat atgaaacggc atgacttttt caagagtgcc
      301 atgcccgaag gttatgtaca ggaaagaact ataagcttca aagatgacgg gaactacaag
      361 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      421 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactgc
      481 gagtccaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagc gaacttcaaa
      541 accagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      601 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac atgctctgcc
      661 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
      721 gctgggatta cacatggcat ggatgaacta tacaaataat aacgatccga gctcgagatc
      781 tgcagccaag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      841 gaactcc
//
