LOCUS       sequence-162179-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Lohman JR, Remington SJ
  TITLE     Development of a family of redox-sensitive green fluorescent protein
            indicators for use in relatively oxidizing subcellular environments.
  JOURNAL   Biochemistry. 2008 Aug 19;47(33):8678-88. doi: 10.1021/bi800498g. 
            Epub 2008 Jul 25.
  PUBMED    18652491
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi800498g";
            journalName: "Biochemistry"; date: "2008-08-19- 19"; volume: "47"; 
            issue: "33"; pages: "8678-88"
COMMENT     Sequence Label: pQE30-roGFP1-iE
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(119..139)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     737..765
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cattactgga tctatcaaca ggagtccaag ctcagctaat taagcttggc tgcagatctc
       61 gagctcggat cgttattatt tgtatagttc atccatgcca tgtgtaatcc cagcagctgt
      121 tacaaactca agaaggacca tgtggtctct cttttcgttg ggatctttcg aaagggcaga
      181 gcatgtggac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt
      241 ttgttgataa tggtctgcta gttgaacgct tccatcttca atgttgtgtc tggttttgaa
      301 gttcgctttg attccattct tttgtttgtc tgccatgatg tatacattgg actcgcagtt
      361 atagttgtat tccaatttgt gtccaagaat gtttccatct tctttaaaat caataccttt
      421 taactcgatt ctattaacaa gggtatcacc ttcaaacttg acttcagcac gtgtcttgta
      481 gttcccgtca tctttgaagc ttatagttct ttcctgtaca taaccttcgg gcatggcact
      541 cttgaaaaag tcatgccgtt tcatatgatc tgggtatctt gaaaagcatt gaacaccata
      601 actcagagta gtgacaagtg ttggccatgg aacaggtagt tttccagtag tggaaataaa
      661 tttaagggta agttttccgt atgttgcatc accttcaccc tctccactga cagaaaattt
      721 gtgcccatta acatcaccat ctaattcaac aagaattggg acaactccag tgaa
//
