LOCUS       sequence-162186-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pentaprobe for assesing the RNA binding potential of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP
  TITLE     A rapid method for assessing the RNA-binding potential of a protein.
  JOURNAL   Nucleic Acids Res. 2012 Aug;40(14):e105. doi: 10.1093/nar/gks285. 
            Epub 2012 Apr 3.
  PUBMED    22492509
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-08"; volume: "40(14):e105. doi"; pages: " 
            10.1093/nar/gks285. Epub 2012 Apr 3"
COMMENT     Sequence Label: Pentaprobe PP2
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(205..223)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(206..223)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(243..260)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    249..473
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      519..947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(606..625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     816..837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(956..976)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac tatcttactt tagtttcatt taattgtgtt
      121 gtactctcct ctgcgttcac ttagcttaac ttggtttggc ttgatttgac ttcagttgcg
      181 ctctattcta ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
      241 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      301 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      361 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
      421 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
      481 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
      541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      661 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      721 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      841 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
      961 gtgtgtcagt tagggtgtgg aaagtccc
//
