LOCUS       sequence-162188-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Lohman JR, Remington SJ
  TITLE     Development of a family of redox-sensitive green fluorescent protein
            indicators for use in relatively oxidizing subcellular environments.
  JOURNAL   Biochemistry. 2008 Aug 19;47(33):8678-88. doi: 10.1021/bi800498g. 
            Epub 2008 Jul 25.
  PUBMED    18652491
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi800498g";
            journalName: "Biochemistry"; date: "2008-08-19- 19"; volume: "47"; 
            issue: "33"; pages: "8678-88"
COMMENT     Sequence Label: pQE30-roGFP1-iR
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(81..797)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCRSNVYITADKQKNGIK
                     ANFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(121..141)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     739..767
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtcattactg gatctatcaa caggagtcca agctcagcta attaagcttg gctgcagatc
       61 tcgagctcgg atcgttatta tttgtatagt tcatccatgc catgtgtaat cccagcagct
      121 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      181 gagcatgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      241 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctggttttg
      301 aagttcgctt tgattccatt cttttgtttg tctgccgtga tgtatacatt cgacctgcag
      361 ttatagttgt attccaattt gtgtccaaga atgtttccat cttctttaaa atcaatacct
      421 tttaactcga ttctattaac aagggtatca ccttcaaact tgacttcagc acgtgtcttg
      481 tagttcccgt catctttgaa gcttatagtt ctttcctgta cataaccttc gggcatggca
      541 ctcttgaaaa agtcatgccg tttcatatga tctgggtatc ttgaaaagca ttgaacacca
      601 taactcagag tagtgacaag tgttggccat ggaacaggta gttttccagt agtggaaata
      661 aatttaaggg taagttttcc gtatgttgca tcaccttcac cctctccact gacagaaaat
      721 ttgtgcccat taacatcacc atctaattca acaagaattg ggacaactcc agtgaaaagt
      781 tcttctcctt tactcatgg
//
