LOCUS       sequence-162203-        4239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DOK1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DOK1_WT
FEATURES             Location/Qualifiers
     source          1..4239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1639..1738)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1755..1774)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1756..1774)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1779..1795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1779..1795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2226..3017
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3110..3698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3599..3618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3852..3869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4028..4055)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4147..4233)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggacgga gcagtgatgg aagggccgct ttttttgcag agtcagcgct
      241 ttgggaccaa gaggtggagg aagacctggg ccgtgctcta cccggccagt ccccacggcg
      301 tagcgcggct cgagttcttt gaccataagg ggtcgagctc tgggggtggc cgagggagct
      361 cgcgccgcct ggactgcaaa gtgatccgtc tggctgagtg tgtgagtgtg gcccccgtca
      421 ccgtggagac cccccctgag cccggcgcca ctgccttccg cctggacact gctcagcgct
      481 cgcacctgct ggcggccgac gcgccgtcca gtgcagcctg ggtgcagacg ctgtgccgaa
      541 acgcctttcc gaaaggcagc tggactctgg cgcctaccga taacccacct aagctttctg
      601 ccctggagat gctggagaac tccttgtaca gccctacctg ggaaggatcc caattctggg
      661 taacggtgca gaggactgag gccgccgagc gctgtggcct gcatggctcc tacgtgctga
      721 gggtggaggc tgaaaggctg actctcctga ccgtgggggc ccagagtcag atactggagc
      781 cactcctgtc ctggccctac actctgttgc gtcgctatgg ccgggacaag gtcatgttct
      841 ctttcgaggc cggccgccgc tgcccctcag gccctggaac cttcaccttc cagacggcac
      901 agggaaatga catcttccag gcagttgaga ctgccatcca ccggcagaag gcccagggaa
      961 aggccggaca ggggcacgat gttctcagag ctgactccca tgaaggggag gtggcagagg
     1021 ggaagttgcc ttccccacct ggcccccaag agctcctcga cagtccccca gccctgtatg
     1081 ctgagccctt agactccctg cgcattgctc catgcccttc ccaggactcc ctatactcag
     1141 accccttgga cagcacgtct gctcaggcag gagagggagt acaacggaag aaacctctct
     1201 attgggactt gtatgagcat gcgcagcagc agttgctgaa ggccaagctg acagacccca
     1261 aagaggatcc catctatgat gaacctgagg gcctggcccc agtccctccc cagggccttt
     1321 atgatctgcc tcgggagccc aaggatgcat ggtggtgcca agctcgggtg aaggaggagg
     1381 gctatgagct cccctacaac cctgccactg atgactacgc tgtgccaccc cctcggagca
     1441 caaagcccct ccttgctccc aagccccagg gcccagcctt ccctgaacct ggtactgcaa
     1501 ctggcagtgg catcaaaagc cacaactcag ccctgtacag ccaggtccag aagagcgggg
     1561 cctcagggag ctgggactgt gggctctcta gagtagggac tgacaagact ggggtcaagt
     1621 cagagggctc tacctgattg ccaactttct tgtacaaagt tggcattata agaaagcatt
     1681 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     1741 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1801 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1861 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1921 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1981 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2041 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2101 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2161 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2221 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2281 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2341 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2401 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2461 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2521 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2581 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2641 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2701 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2761 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2821 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2881 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2941 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3001 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3061 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3241 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3361 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3481 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3721 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3781 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3841 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3901 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3961 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4021 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4081 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4141 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4201 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
