LOCUS       sequence-162204-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GFP tagged mutant BuGZ in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Toledo CM, Herman JA, Olsen JB, Ding Y, Corrin P, Girard EJ, Olson 
            JM, Emili A, DeLuca JG, Paddison PJ
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and 
            chromosome alignment.
  JOURNAL   Dev Cell. 2014 Feb 10;28(3):282-94. doi: 
            10.1016/j.devcel.2013.12.014. Epub 2014 Jan 23.
  PUBMED    24462187
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.12.014"; journalName: "Dev Cell"; date: 
            "2014-02-10- 10"; volume: "28"; issue: "3"; pages: "282-94"
COMMENT     Sequence Label: GUT BuGZ delta ZF1
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..741
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
ORIGIN
        1 tcggcgcgcc agtcctccga cagactgagt cgcccgctgg agaccatgga gagcgacgag
       61 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
      121 ttcgagctgg tgggcggcgg agagggcacc cccgagcagg gccgcatgac caacaagatg
      181 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
      241 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
      301 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
      361 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtgatgggc
      421 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
      481 gtggagcacc tgcaccccat gggcgataac gatctggatg gcagcttcac ccgcaccttc
      541 agcctgcgcg acggcggcta ctacagctcc gtggtggaca gccacatgca cttcaagagc
      601 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
      661 gaggatcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccg
      721 gatgcagatg ccggtgaaga atacatgggt cgcaagaaga agaagcagct gaagccgtgg
      781 tt
//
