LOCUS       sequence-162222-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split GFP assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Kodaka M, Yang Z, Nakagawa K, Maruyama J, Xu X, Sarkar A, Ichimura 
            A, Nasu Y, Ozawa T, Iwasa H, Ishigami-Yuasa M, Ito S, Kagechika H, 
            Hata Y
  TITLE     A new cell-based assay to evaluate myogenesis in mouse myoblast 
            C2C12 cells.
  JOURNAL   Exp Cell Res. 2015 Aug 15;336(2):171-81. doi: 
            10.1016/j.yexcr.2015.06.015. Epub 2015 Jun 24.
  PUBMED    26116467
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2015.06.015"; journalName: "Exp Cell Res"; date: 
            "2015-08-15- 15"; volume: "336"; issue: "2"; pages: "171-81"
COMMENT     Sequence Label: pQCXIP-GFP1-10
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        60..363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        364..567
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     518..538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     564..588
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(754..775)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaattg aagatctggg ggatcgatcc tctagagtcc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      541 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      601 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
      661 gaacggtgca ttggaacgga cctgcaggaa ttgatccgcg gccgatccat ggtgagcaag
      721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      781 ggccacaagt tcagcgtgcg cggcgagggc ga
//
