LOCUS       sequence-162235-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pentaprobe for assesing the RNA binding potential of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP
  TITLE     A rapid method for assessing the RNA-binding potential of a protein.
  JOURNAL   Nucleic Acids Res. 2012 Aug;40(14):e105. doi: 10.1093/nar/gks285. 
            Epub 2012 Apr 3.
  PUBMED    22492509
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-08"; volume: "40(14):e105. doi"; pages: " 
            10.1093/nar/gks285. Epub 2012 Apr 3"
COMMENT     Sequence Label: Pentaprobe PP8
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(139..158)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(140..158)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(203..406)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(232..252)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        407..786
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctatagaata gggccctcta gtatcttact ttagtttcat ttaattgtgt tgtactctcc
       61 tctgcgttca cttagcttaa cttggtttgg cttgatttga cttcagttgc gctctattct
      121 agtaccaagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
      181 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
      241 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
      301 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
      361 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
      421 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
      481 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
      541 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
      601 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
      661 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
      721 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
      781 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
      841 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
      901 cgcactactc agcgacctcc a
//
