LOCUS       sequence-162238-        6555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ERBB2_p.D845A
FEATURES             Location/Qualifiers
     source          1..6555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3955..4054)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4071..4090)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4072..4090)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4095..4111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4095..4111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4542..5333
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5426..6014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5915..5934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6168..6185
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6344..6371)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6463..6549)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggagctggcg gccttgtgcc gctgggggct cctcctcgcc ctcttgcccc
      241 ccggagccgc gagcacccaa gtgtgcaccg gcacagacat gaagctgcgg ctccctgcca
      301 gtcccgagac ccacctggac atgctccgcc acctctacca gggctgccag gtggtgcagg
      361 gaaacctgga actcacctac ctgcccacca atgccagcct gtccttcctg caggatatcc
      421 aggaggtgca gggctacgtg ctcatcgctc acaaccaagt gaggcaggtc ccactgcaga
      481 ggctgcggat tgtgcgaggc acccagctct ttgaggacaa ctatgccctg gccgtgctag
      541 acaatggaga cccgctgaac aataccaccc ctgtcacagg ggcctcccca ggaggcctgc
      601 gggagctgca gcttcgaagc ctcacagaga tcttgaaagg aggggtcttg atccagcgga
      661 acccccagct ctgctaccag gacacgattt tgtggaagga catcttccac aagaacaacc
      721 agctggctct cacactgata gacaccaacc gctctcgggc ctgccacccc tgttctccga
      781 tgtgtaaggg ctcccgctgc tggggagaga gttctgagga ttgtcagagc ctgacgcgca
      841 ctgtctgtgc cggtggctgt gcccgctgca aggggccact gcccactgac tgctgccatg
      901 agcagtgtgc tgccggctgc acgggcccca agcactctga ctgcctggcc tgcctccact
      961 tcaaccacag tggcatctgt gagctgcact gcccagccct ggtcacctac aacacagaca
     1021 cgtttgagtc catgcccaat cccgagggcc ggtatacatt cggcgccagc tgtgtgactg
     1081 cctgtcccta caactacctt tctacggacg tgggatcctg caccctcgtc tgccccctgc
     1141 acaaccaaga ggtgacagca gaggatggaa cacagcggtg tgagaagtgc agcaagccct
     1201 gtgcccgagt gtgctatggt ctgggcatgg agcacttgcg agaggtgagg gcagttacca
     1261 gtgccaatat ccaggagttt gctggctgca agaagatctt tgggagcctg gcatttctgc
     1321 cggagagctt tgatggggac ccagcctcca acactgcccc gctccagcca gagcagctcc
     1381 aagtgtttga gactctggaa gagatcacag gttacctata catctcagca tggccggaca
     1441 gcctgcctga cctcagcgtc ttccagaacc tgcaagtaat ccggggacga attctgcaca
     1501 atggcgccta ctcgctgacc ctgcaagggc tgggcatcag ctggctgggg ctgcgctcac
     1561 tgagggaact gggcagtgga ctggccctca tccaccataa cacccacctc tgcttcgtgc
     1621 acacggtgcc ctgggaccag ctctttcgga acccgcacca agctctgctc cacactgcca
     1681 accggccaga ggacgagtgt gtgggcgagg gcctggcctg ccaccagctg tgcgcccgag
     1741 ggcactgctg gggtccaggg cccacccagt gtgtcaactg cagccagttc cttcggggcc
     1801 aggagtgcgt ggaggaatgc cgagtactgc aggggctccc cagggagtat gtgaatgcca
     1861 ggcactgttt gccgtgccac cctgagtgtc agccccagaa tggctcagtg acctgttttg
     1921 gaccggaggc tgaccagtgt gtggcctgtg cccactataa ggaccctccc ttctgcgtgg
     1981 cccgctgccc cagcggtgtg aaacctgacc tctcctacat gcccatctgg aagtttccag
     2041 atgaggaggg cgcatgccag ccttgcccca tcaactgcac ccactcctgt gtggacctgg
     2101 atgacaaggg ctgccccgcc gagcagagag ccagccctct gacgtccatc atctctgcgg
     2161 tggttggcat tctgctggtc gtggtcttgg gggtggtctt tgggatcctc atcaagcgac
     2221 ggcagcagaa gatccggaag tacacgatgc ggagactgct gcaggaaacg gagctggtgg
     2281 agccgctgac acctagcgga gcgatgccca accaggcgca gatgcggatc ctgaaagaga
     2341 cggagctgag gaaggtgaag gtgcttggat ctggcgcttt tggcacagtc tacaagggca
     2401 tctggatccc tgatggggag aatgtgaaaa ttccagtggc catcaaagtg ttgagggaaa
     2461 acacatcccc caaagccaac aaagaaatct tagacgaagc atacgtgatg gctggtgtgg
     2521 gctccccata tgtctcccgc cttctgggca tctgcctgac atccacggtg cagctggtga
     2581 cacagcttat gccctatggc tgcctcttag accatgtccg ggaaaaccgc ggacgcctgg
     2641 gctcccagga cctgctgaac tggtgtatgc agattgccaa ggggatgagc tacctggagg
     2701 atgtgcggct cgtacacagg gcattggccg ctcggaacgt gctggtcaag agtcccaacc
     2761 atgtcaaaat tacagacttc gggctggctc ggctgctgga cattgacgag acagagtacc
     2821 atgcagatgg gggcaaggtg cccatcaagt ggatggcgct ggagtccatt ctccgccggc
     2881 ggttcaccca ccagagtgat gtgtggagtt atggtgtgac tgtgtgggag ctgatgactt
     2941 ttggggccaa accttacgat gggatcccag cccgggagat ccctgacctg ctggaaaagg
     3001 gggagcggct gccccagccc cccatctgca ccattgatgt ctacatgatc atggtcaaat
     3061 gttggatgat tgactctgaa tgtcggccaa gattccggga gttggtgtct gaattctccc
     3121 gcatggccag ggacccccag cgctttgtgg tcatccagaa tgaggacttg ggcccagcca
     3181 gtcccttgga cagcaccttc taccgctcac tgctggagga cgatgacatg ggggacctgg
     3241 tggatgctga ggagtatctg gtaccccagc agggcttctt ctgtccagac cctgccccgg
     3301 gcgctggggg catggtccac cacaggcacc gcagctcatc taccaggagt ggcggtgggg
     3361 acctgacact agggctggag ccctctgaag aggaggcccc caggtctcca ctggcaccct
     3421 ccgaaggggc tggctccgat gtatttgatg gtgacctggg aatgggggca gccaaggggc
     3481 tgcaaagcct ccccacacat gaccccagcc ctctacagcg gtacagtgag gaccccacag
     3541 tacccctgcc ctctgagact gatggctacg ttgcccccct gacctgcagc ccccagcctg
     3601 aatatgtgaa ccagccagat gttcggcccc agcccccttc gccccgagag ggccctctgc
     3661 ctgctgcccg acctgctggt gccactctgg aaaggcccaa gactctctcc ccagggaaga
     3721 atggggtcgt caaagacgtt tttgcctttg ggggtgccgt ggagaacccc gagtacttga
     3781 caccccaggg aggagctgcc cctcagcccc accctcctcc tgccttcagc ccagccttcg
     3841 acaacctcta ttactgggac caggacccac cagagcgggg ggctccaccc agcaccttca
     3901 aagggacacc tacggcagag aacccagagt acctgggtct ggacgtgcca gtgtgaccaa
     3961 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     4021 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     4081 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     4141 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     4201 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     4261 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     4321 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     4381 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     4441 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     4501 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     4561 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     4621 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     4681 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     4741 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     4801 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     4861 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     4921 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     4981 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     5041 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     5101 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     5161 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     5221 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     5281 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     5341 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     5401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6001 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6301 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     6361 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     6421 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     6481 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     6541 ttttatttga tgcct
//
